Production and characterization of protective human antibodies against shiga toxin 1

被引：50

作者：

Mukherjee, J

Chios, K

Fishwild, D

Hudson, D

O'Donnell, S

Rich, SM

Donohue-Rolfe, A

Tzipori, S

机构：

[1] Tufts Univ, Sch Vet Med, Div Infect Dis, North Grafton, MA 01536 USA

[2] Medarex, San Jose, CA USA

来源：

INFECTION AND IMMUNITY | 2002年 / 70卷 / 10期

关键词：

D O I：

10.1128/IAI.70.10.5896-5899.2002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Hemolytic-uremic syndrome (HUS) is a serious complication which is predominantly associated in children with infection by Shiga toxin-producing Escherichia coli (STEC). By using HuMAb-Mouse (Medarex) animals, human monoclonal antibodies (Hu-MAbs) were developed against Shiga toxin 1 (Stx1) for passive immunotherapy of HUS. Ten stable hybridomas comprised of fully human heavy- and light-chain immunoglobulin elements and secreting Stx1-specific Hu-MAbs (seven immunoglobulin M(kappa) [IgM(kappa)] elements [one specific for the A subunit and six specific for the B subunit] and three IgG1(kappa) elements specific for subunit B) were isolated. Two IgM(kappa) Hu-MAbs (2D9 and 15G9) and three IgG1(kappa) Hu-MAbs (5A4, 10F4, and 15G2), all specific for subunit B, demonstrated marked neutralization of Stx1 in vitro and significant prolongation of survival in a murine model of Stx1 toxicosis.

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页码：5896 / 5899

页数：4

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