Development of cell-selective films for layered co-culturing of vascular progenitor cells

被引:33
作者
Chong, Mark S. K. [1 ,2 ,3 ]
Chan, Jerry [2 ]
Choolani, Mahesh [2 ]
Lee, Chuen-Neng [3 ]
Teoh, Swee-Hin [1 ]
机构
[1] Natl Univ Singapore, Ctr Biomed Mat Applicat & Technol BIOMAT, Dept Mech Engn, Singapore 117576, Singapore
[2] Natl Univ Singapore, Expt Fetal Med Grp, Dept Obstet & Gynaecol, Yong Loo Lin Sch Med, Singapore 119074, Singapore
[3] Natl Univ Singapore, Dept Surg, Yong Loo Lin Sch Med, Singapore 119074, Singapore
关键词
Polycaprolactone; Surface modification; Cell capture; Micro-thin films; Cell sheet engineering; SMOOTH-MUSCLE-CELLS; ACRYLIC-ACID; ENDOTHELIAL-CELLS; SURFACE MODIFICATION; POLY(ACRYLIC ACID); TISSUE; COLLAGEN; GROWTH; IMMOBILIZATION; SCAFFOLD;
D O I
10.1016/j.biomaterials.2008.12.056
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Cell-sheet assemblies are currently being studied for tissue engineering. However, tissues engineered from completely biological cell sheets lack substrate cues and possess poor mechanical strength. Recent studies demonstrate the use of synthetic bioresorbable films as scaffolds that may address these issues. Here, we describe the application of a micro-thin, biaxially-stretched polycaprolactone (mu XPCL) with surface modifications for layered tissue engineering, and present the results of biphasic cell-sheet constructs using surfaces optimised for specific cell types. Polyacrylic acid (PAAc) was grafted onto mu XPCL film surfaces by low-pressure plasma immobilisation. This provided a surface suitable for perivascular cells, forming the medial compartment. Subsequently, endothelial progenitor cell (EPC)-selective CD34 antibody was conjugated onto the reverse surface (intimal compartment) to select and anchor EPCs for improved adhesion and proliferation. Using the blood vessel as a model, a biphasic culture system was then setup to represent a tunica intima (endothelial cells) and tunica media (smooth muscle cells). When suitable cell types were cultured in the corresponding compartments, confluent layers of the respective populations were achieved distinctively from each other. These results demonstrate the use of mu XPCL films with cell-selective modifications for layered co-cultures towards the generation of stratified tissue. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:2241 / 2251
页数:11
相关论文
共 47 条
[1]   Cotton fabric graft copolymerization using microwave plasma. I. Universal attenuated total reflectance-FTIR study [J].
Abidi, N ;
Hequet, E .
JOURNAL OF APPLIED POLYMER SCIENCE, 2004, 93 (01) :145-154
[2]   Characterization of porcine circulating progenitor cells: Toward a functional endothelium [J].
Allen, Josephine ;
Khan, Sadiya ;
Serrano, Maria Concepcion ;
Ameer, Guillermo .
TISSUE ENGINEERING PART A, 2008, 14 (01) :183-194
[3]   Endothelial progenitor cell capture by stents coated with antibody against CD34 - The HEALING-FIM (healthy endothelial accelerated lining inhibits neointimal growth-first in man) registry [J].
Aoki, J ;
Serruys, PW ;
van Beusekom, H ;
Ong, ATL ;
McFadden, EP ;
Sianos, G ;
van der Giessen, WJ ;
Regar, E ;
de Feyter, PJ ;
Davis, HR ;
Rowland, S ;
Kutryk, MJB .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2005, 45 (10) :1574-1579
[4]   Isolation of putative progenitor endothelial cells for angiogenesis [J].
Asahara, T ;
Murohara, T ;
Sullivan, A ;
Silver, M ;
vanderZee, R ;
Li, T ;
Witzenbichler, B ;
Schatteman, G ;
Isner, JM .
SCIENCE, 1997, 275 (5302) :964-967
[5]   Basic science review: The helix and the heart [J].
Buckberg, GD .
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY, 2002, 124 (05) :863-883
[6]   Plasma-surface modification of biomaterials [J].
Chu, PK ;
Chen, JY ;
Wang, LP ;
Huang, N .
MATERIALS SCIENCE & ENGINEERING R-REPORTS, 2002, 36 (5-6) :143-206
[7]   Fine-tuning of a three-dimensional microcarrier-based angiogenesis assay for the analysis of endothelial-mesenchymal cell co-cultures in fibrin and collagen gels [J].
Dietrich F. ;
Lelkes P.I. .
Angiogenesis, 2006, 9 (3) :111-125
[8]   Growth factors upregulate deposition and remodeling of ECM by endothelial cells cultured for tissue-engineering applications [J].
Divya, P. ;
Sreerekha, P. R. ;
Krishnan, Lissy K. .
BIOMOLECULAR ENGINEERING, 2007, 24 (06) :593-602
[9]   3D hepatocyte monolayer on hybrid RGD/galactose substratum [J].
Du, Yanan ;
Chia, Ser-mien ;
Han, Rongbin ;
Chang, Shi ;
Tang, Huihuan ;
Yu, Hanry .
BIOMATERIALS, 2006, 27 (33) :5669-5680
[10]   Tethered epidermal growth factor provides a survival advantage to mesenchymal stem cells [J].
Fan, Vivian H. ;
Au, Ada ;
Tamama, Kenichi ;
Littrell, Romie ;
Richardson, Llewellyn B. ;
Wright, John W. ;
Wells, Alan ;
Griffith, Linda G. .
STEM CELLS, 2007, 25 (05) :1241-1251