Hemifusion arrest by complexin is relieved by Ca2+-synaptotagmin I

被引：172

作者：

Schaub, Johanna R.

Lu, Xiaobing

Doneske, Blair

Shin, Yeon-Kyun

McNew, James A.

机构：

[1] Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77005 USA

[2] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2006年 / 13卷 / 08期

关键词：

D O I：

10.1038/nsmb1124

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Synaptic transmission relies on an exquisitely orchestrated series of protein-protein interactions. Here we show that fusion driven by neuronal SNAREs is inhibited by the regulatory protein complexin. Furthermore, inner-leaflet mixing is strongly impaired relative to total lipid mixing, indicating that inhibition by complexin arrests fusion at hemifusion. When the calcium sensor synaptotagmin is added in the presence of calcium, inhibition by complexin is relieved and full fusion rapidly proceeds.

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页码：748 / 750

页数：3

相关论文

共 20 条

[1] Complexin regulates the closure of the fusion pore during regulated vesicle exocytosis [J].

Archer, DA ;

Graham, ME ;

Burgoyne, RD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (21) :18249-18252

[2] The C2 domains of synaptotagmin - partners in exocytosis [J].

Bai, JH ;

Chapman, ER .

TRENDS IN BIOCHEMICAL SCIENCES, 2004, 29 (03) :143-151

[3] Synaptotagmin isoforms couple distinct ranges of Ca2+, Ba2+, and Sr2+ concentration to SNARE-mediated membrane fusion [J].

Bhalla, A ;

Tucker, WC ;

Chapman, ER .

MOLECULAR BIOLOGY OF THE CELL, 2005, 16 (10) :4755-4764

[4] Ca2+-synaptotagmin directly regulates t-SNARE function during reconstituted membrane fusion [J].

Bhalla, A ;

Chicka, MC ;

Tucker, WC ;

Chapman, ER .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2006, 13 (04) :323-330

[5] X-ray structure of a neuronal complexin-SNARE complex from squid [J].

Bracher, A ;

Kadlec, J ;

Betz, H ;

Weissenhorn, W .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (29) :26517-26523

[6] Three-dimensional structure of the complexin/SNARE complex [J].

Chen, XC ;

Tomchick, DR ;

Kovrigin, E ;

Araç, D ;

Machius, M ;

Südhof, TC ;

Rizo, J .

NEURON, 2002, 33 (03) :397-409

[7] Membrane hemifusion: Crossing a chasm in two leaps [J].

Chernomordik, LV ;

Kozlov, MM .

CELL, 2005, 123 (03) :375-382

[8] FlyBase: genes and gene models [J].

Drysdale, RA ;

Crosby, MA .

NUCLEIC ACIDS RESEARCH, 2005, 33 :D390-D395

[9] SNAREs can promote complete fusion and hemifusion as alternative outcomes [J].

Giraudo, CG ;

Hu, C ;

You, DQ ;

Slovic, AM ;

Mosharov, EV ;

Sulzer, D ;

Melia, TJ ;

Rothman, JE .

JOURNAL OF CELL BIOLOGY, 2005, 170 (02) :249-260

[10] Transfection analysis of functional roles of complexin I and II in the exocytosis of two different types of secretory vesicles [J].

Itakura, M ;

Misawa, H ;

Sekiguchi, M ;

Takahashi, S ;

Takahashi, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 265 (03) :691-696