Amino terminal peptides of the ring infected erythrocyte surface antigen of Plasmodium falciparum bind specifically to erythrocytes

被引：26

作者：

Bravo, RV ^{[1
]}

Marín, V ^{[1
]}

García, J ^{[1
]}

Urquiza, M ^{[1
]}

Torres, E ^{[1
]}

Trujillo, M ^{[1
]}

Rosas, J ^{[1
]}

Patarroyo, ME ^{[1
]}

机构：

[1] Univ Nacl Colombia, Hosp San Juan de Dios, Inst Inmunol, Santafe De Bogota, Colombia

来源：

VACCINE | 2000年 / 18卷 / 14期

关键词：

Pf155/RESA; Plasmodium falciparum; erythrocyte-binding;

D O I：

10.1016/S0264-410X(99)00405-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The Ring-Infected Erythrocyte Surface Antigen (Pf155/RESA) sequence was chemically synthesized: in fifty four 20-mer sequential peptides, covering the entire protein, each of which was tested in erythrocyte binding assays. Peptides 6671 and 6673, corresponding to residues 141-160 and 181-200, respectively, presented a high specific binding activity to erythrocytes with affinity constants of 190 nM and 105 nM respectively. Their binding was sensitive to previous enzymatic treatment of erythrocytes. A region of peptide 6673 has been identified, very recently, as a B-cell epitope, target of neutralizing antibodies (Siddique AB, Iqbal J, Ahlborg N, Wahlin FB, Perlmann P, Berzins K. Antibodies to nonrepeat sequences Of antigen Pf155/ RESA of Plasmodium falciparum inhibit parasite growth in vitro. Parasitol Res 1998;84:485-91). The critical residues for erythrocyte binding for peptide 6671 (MTDVNR YR YSNNYEAIPHIS) and for peptide 6673 (LGRSGGDI/KKMQTLWDEIM) were recognized. Based on these data, the presence of five functional regions of RESA is postulated. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：1289 / 1293

页数：5

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