Transforming growth factor-β1 induced alteration of skeletal morphogenesis in vivo

被引：27

作者：

Ferguson, CM ^{[1
]}

Schwarz, EM ^{[1
]}

Puzas, JE ^{[1
]}

Zuscik, MJ ^{[1
]}

Drissi, H ^{[1
]}

O'Keefe, RJ ^{[1
]}

机构：

[1] Univ Rochester, Sch Med & Dent, Ctr Musculoskeletal Res, Dept Orthopaed, Rochester, NY 14642 USA

来源：

JOURNAL OF ORTHOPAEDIC RESEARCH | 2004年 / 22卷 / 04期

关键词：

transforming growth factor-beta; chondrocyte biology; chondrocyte maturation; embryonic limb development; chondrocyte proliferation;

D O I：

10.1016/j.orthres.2003.10.023

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Transforming growth factor beta (TGF-beta) is expressed in the growth plate and is an important regulator of chondrocyte maturation. Loss of function results in premature chondrocyte maturation both in vitro and in vivo. While TGF-beta inhibits chondrocyte maturation in cell cultures, the effect of increased TGF-beta has not been well characterized in an ill vivo development model. Addition of Affi-gel agarose beads loaded with TGF-beta1 (10 ng/mul) to developing stage 24-25 chick limb buds resulted in limb shortening and altered morphology. In situ hybridization studies showed down regulation of Indian hedgehog (ihh), bone morphogenetic protein 6 (bmp6), and collagen type X (colX) expression, markers of chondrocyte maturation, in TGF-beta1 treated limbs. TGF-beta1 also decreased chondrocyte proliferation in the developing anlage. The findings confirm a critical role for TGF-beta during skeletal development. A more complete understanding of the role of TGF-beta and its down-stream signals will lead to improved understanding and treatment of cartilage diseases. (C) 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.

引用

页码：687 / 696

页数：10

共 41 条

[1] The perichondrium plays an important role in mediating the effects of TGF-β1 on endochondral bone formation [J].