Vaccination with inactivated murine gammaherpesvirus 68 strongly limits viral replication and latency and protects type I IFN receptor knockout mice from a lethal infection

被引:11
作者
Aricò, E
Robertson, KA
Belardelli, F
Ferrantini, M
Nash, AA
机构
[1] Ist Super Sanita, Virol Lab, I-10061 Rome, Italy
[2] Univ Edinburgh, Dept Vet Pathol, Edinburgh EH9 1QH, Midlothian, Scotland
关键词
B cell; MHV-68; type IIFN;
D O I
10.1016/j.vaccine.2003.10.015
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Human gammaherpesviruses such as Epstein-Barr virus (EBV) cause lifelong infections and associated diseases, including malignancies, and the development of an effective vaccine against this class of viral infections is of considerable interest. The murine herpesvirus 68 (MHV-68) model provides a useful experimental setting to investigate the immune response to gammaherpesvirus infections and to evaluate the efficacy of vaccination strategies. In this study, we tested a heat-inactivated MHV-68 vaccine in immunocompetent mice as well as in B cell-deficient or type I IFN receptor knockout mice. Vaccination with heat-inactivated MHV-68 protected immunocompetent mice from the acute MHV-68 infection in the lung and strongly reduced the expansion of latently infected cells in the spleen and the development of splenomegaly. A similar inhibition of the acute viral replication in the lung was also observed in vaccinated B cell-deficient mice. Of note, the inactivated MHV-68 vaccine completely protected type I IFN receptor knockout mice from the infection with a lethal dose of MHV-68. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1433 / 1440
页数:8
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