Different mechanisms for testosterone-induced relaxation of aorta between normotensive and spontaneously hypertensive rats

被引:123
作者
Honda, H [1 ]
Unemoto, T [1 ]
Kogo, H [1 ]
机构
[1] Tokyo Univ Pharm & Life Sci, Dept Pharmacol, Hachioji, Tokyo 1930392, Japan
关键词
testosterone; endothelium; rats; inbred SHR; inbred WKY; aorta; potassium channels;
D O I
10.1161/01.HYP.34.6.1232
中图分类号
R6 [外科学];
学科分类号
1002 ; 100210 ;
摘要
The tension in isolated ring preparations of the thoracic aortae from Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) was measured isometrically to study the differences in testosterone-induced relaxation between WKY and SHR aortic rings. Testosterone (9 to 300 mu mol/L) induced a concentration-dependent relaxation in both WKY and SHR aortic rings, and the relaxation induced by testosterone was greater in SHR than WKY. The relaxation induced by testosterone was significantly reduced by denudation of endothelium in SHR but not WKY. Indomethacin, an inhibitor of cyclooxygenase, and N-G-nitro-L-arginine, an inhibitor of nitric oxide (NO) synthase, showed Little influence on the relaxation induced by testosterone in both WKY and SHR aortic rings. Glibendamide, a selective inhibitor of ATP-sensitive potassium channels, significantly reduced the relaxation induced by testosterone in both WKY and SHR aortic rings, although the extent of reduction was greater in WKY than SHR. On the other hand, 4-aminopyridine, a selective inhibitor of voltage-dependent potassium channels, and tetraethylammonium, an inhibitor of calcium-activated potassium channels, significantly reduced the relaxation induced by testosterone in SHR but not WKY. These results suggest that the mechanisms of testosterone-induced vasorelaxation in both WKY and SHR involve, in part, ATP-sensitive potassium channels in the thoracic aortae and that in SHR aortic rings, testosterone may release endothelium-derived substances that may cause hyperpolarization of the cells by a mechanism that involves potassium channels. Moreover, the data show differences between WKY and SHR in the function of ATP-sensitive, voltage-dependent, and calcium-activated potassium channels.
引用
收藏
页码:1232 / 1236
页数:5
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