Immunotoxic catecholamine lesions attenuate 2DG-induced increase of AGRP mRNA

被引：26

作者：

Fraley, GS ^{[1
]}

Dinh, TT ^{[1
]}

Ritter, S ^{[1
]}

机构：

[1] Washington State Univ, Dept Vet & Comparat Anat Pharmacol & Physiol, Coll Vet Med, Pullman, WA 99164 USA

来源：

PEPTIDES | 2002年 / 23卷 / 06期

关键词：

2-deoxy-D-glucose; norepinephrine; epinephrine; Hindbrain afferents; paraventricular nucleus of the hypothalamus; arcuate nucleus; anti-dopamine-beta-hydroxylase saporin; glucoprivic feeding;

D O I：

10.1016/S0196-9781(02)00044-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Injections of the immunotoxin, saporin conjugated to anti-dopan-tine-p-hydroxylase (I)SAP), into the hypothalamic paraventricular nucleus (PVH) selectively destroy norepinephrine (NE) and epinephrine (E) terminals in the medial hypothalamus and abolish glucoprivic feeding. We utilized PVH DSAP injections to examine the role of NE/E neurons in the previously reported 2-deoxy-D-glucose (2DG)-induced increases in mRNA levels for the orexigenic peptides, AGRP and NPY Northern blot analysis revealed that DSAP lesions elevated basal but blocked 2DG-induced increases in AGRP mRNA levels. Changes in NPY mRNA were not detectable. AGRP neurons may contribute to circuitry activated by NE/E neurons for elicitation of glucoregulatory responses. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：1093 / 1099

页数：7

共 37 条

[1] NEUROPEPTIDE-Y IN THE ARCUATE NUCLEUS IS MODULATED BY ALTERATIONS IN GLUCOSE-UTILIZATION [J].