Real-time quantitative PCR detection of genetically modified maximizer maize and roundup ready soybean in some representative foods

被引：207

作者：

Vaïtilingom, M ^{[1
]}

Pijnenburg, H ^{[1
]}

Gendre, F ^{[1
]}

Brignon, P ^{[1
]}

机构：

[1] Tepral, Danone Grp, Beverage Div, Res Ctr, F-67037 Strasbourg, France

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1999年 / 47卷 / 12期

关键词：

Maximizer maize "event 176; Roundup Ready" soybean; real-time quantitative PCR; GMO detection;

D O I：

10.1021/jf981208v

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A fast and quantitative method was developed to detect transgenic "Maximizer" maize "event 176" (Novartis) and "Roundup Ready" soybean (Monsanto) in food by real-time quantitative PCR. The use of the ABI Prism 7700 sequence detection system allowed the determination of the amplified product accumulation through a fluorogenic probe (TaqMan). Fluorescent dyes were chosen in such a way as to coamplify total and transgenic DNA in the same tube. Using real-time quantitative PCR, 2 pg of transgenic or total DNA per gram of starting sample was detected in 3 h after DNA extraction and the relative amounts of "Maximizer" maize and "Roundup Ready" soybean in some representative food products were quantified.

引用

页码：5261 / 5266

页数：6

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