Distribution and evolution of ferripyoverdine receptors in Pseudomonas aeruginosa

被引:46
作者
Bodilis, Josselin [2 ]
Ghysels, Bart [1 ]
Osayande, Julie [1 ]
Matthijs, Sandra [1 ]
Pirnay, Jean-Paul [3 ]
Denayer, Sarah [1 ]
De Vos, Daniel [3 ]
Cornelis, Pierre [1 ]
机构
[1] Vrije Univ Brussel VIB, Lab Microbial Interact, Dept Mol & Cellular Interact, B-1050 Brussels, Belgium
[2] Univ Rouen, UMR CNRS 6143, UFR Sci, Microbiol Grp,Lab M2C,Batiment IRESE B, F-76821 Mont St Aignan, France
[3] Queen Astrid Mil Hosp, Lab Mol & Cellular Technol, Burn Wound Ctr, B-1120 Brussels, Belgium
关键词
MEDIATED IRON UPTAKE; FLUORESCENT PSEUDOMONADS; MOLECULAR-BIOLOGY; GENOME SEQUENCE; UPTAKE SYSTEMS; SIDEROPHORES; IDENTIFICATION; SELECTION; FPVA;
D O I
10.1111/j.1462-2920.2009.01932.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
P>Pseudomonas aeruginosa is a ubiquitous Gram-negative bacterium, which is also able to cause severe opportunistic infections in humans. The colonization of the host is importantly affected by the production of the high-affinity iron (III) scavenging peptidic siderophore pyoverdine. The species P. aeruginosa can be divided into three subgroups ('siderovars'), each characterized by the production of a specific pyoverdine and receptor (FpvA). We used a multiplex PCR to determine the FpvA siderovar on 345 P. aeruginosa strains from environmental or clinical origin. We found about the same proportion of each type in clinical strains, while FpvA type I was slightly over-represented (49%) in environmental strains. Our multiplex PCR also detected the presence or absence of an additional receptor for type I pyoverdine (FpvB). The fpvB gene was in fact present in the vast majority of P. aeruginosa strains (93%), regardless of their siderovar or their origin. Finally, molecular analyses of fpvA and fpvB genes highlighted a complex evolutionary history, probably linked to the central role of iron acquisition in the ecology and virulence of P. aeruginosa.
引用
收藏
页码:2123 / 2135
页数:13
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