Evaluation of sequential multiplex PCR for direct detection of multiple serotypes of Streptococcus pneumoniae from nasopharyngeal secretions

被引:19
作者
Antonio, Martin [1 ]
Hakeem, Ishrat [1 ]
Sankareh, Kawsu [1 ]
Cheung, Yin Bun [2 ]
Adegbola, Richard A. [1 ]
机构
[1] MRC Labs, Bacterial Dis Programme, Banjul, Gambia
[2] London Sch Hyg & Trop Med, Dept Infect & Trop Dis, London WC1, England
关键词
DETERMINING CAPSULAR SEROTYPES; INVASIVE PNEUMOCOCCAL DISEASE; CONJUGATE VACCINE; GAMBIAN CHILDREN; OTITIS-MEDIA; CARRIAGE; IMPACT; IMMUNOGENICITY; EPIDEMIOLOGY; BACTEREMIA;
D O I
10.1099/jmm.0.006031-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Sequential multiplex PCR was evaluated for detection of multiple Streptococcus pneumoniae serotypes directly from nasopharyngeal secretions. A total of 279 nasopharyngeal swab samples were tested blindly. When limited to the 29 serotypes identifiable by the molecular method, the mean number of serotypes identified by the conventional latex/Quellung method was 0.85, which was significantly lower than that by the molecular method (P < 0.0001). The multiplex PCR method identified significantly more serotypes than the latex/Quellung method if limited to the 29 serotypes (P = 0.001 and P = 0.014, respectively).
引用
收藏
页码:296 / 302
页数:7
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