Selective fraction of Atractylodes lancea (Thunb.) DC. and its growth inhibitory effect on human gastric cancer cells

被引：50

作者：

Zhao, Ming ^{[1
]}

Wang, Qingqing ^{[1
]}

Ouyang, Zhen ^{[1
]}

Han, Bangxing ^{[1
]}

Wang, Wenbing ^{[1
]}

Wei, Yuan ^{[1
]}

Wu, Yan ^{[1
]}

Yang, Bing ^{[1
]}

机构：

[1] Jiangsu Univ, Sch Pharmaceut Sci, Zhenjiang 212013, Jiangsu, Peoples R China

来源：

CYTOTECHNOLOGY | 2014年 / 66卷 / 02期

关键词：

Atractylodes lancea (Thunb.) DC; Water extracts; PE fraction; Gastric cells; Growth inhibitory; EXPERIMENTAL STOMACH ULCER; CRUDE DRUG ZHU; ATRACTYLENOLIDE-I; QUALITY; ORIGIN; SESQUITERPENOIDS; CONSTITUENTS; RHIZOMES; EXTRACT;

D O I：

10.1007/s10616-013-9559-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

The purpose of this study was to investigate the pharmacological effect of fraction of Atractylodes lancea (Thunb.) DC. (A. lancea) extract. In this study, we isolated different polarity fractions, including petroleum ether (PE), ethyl acetate, n-butanol, and the remaining H2O fractions from the water extract of A. lancea. The antigastric cancer properties of the different fractions in BGC-823 and SGC-7901 cells were evaluated. Apoptotic cells were treated with PE fraction and stained with Hoechst 33342 and 5,5,6,6-tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide. The cell cycle was analyzed via flow cytometry. The main compounds of PE fraction were determined by HPLC-ESI-MS. Results of this study showed that the PE fraction of A. lancea inhibited the growth of BGC-823 and SGC7901 cells in a dose- and time-dependent manner. The morphological and mitochondrial transmembrane potential changes suggested that the cells showed preliminary apoptosis characteristics after treatment with the three different polarities. The main compounds of PE fraction include two sesquiterpene compounds: eudesm-4(15),7-diene-9 alpha,11-diol and eudesm-4(15)-ene-7a,11-diol; three sesquiterpene lactone compounds: atractylenolid I, atractylenolid III and 3-beta-acetyl-atractylenolid III and one polyacetylenic compound: 4,6,12-tetradecatriene-8,10-diyne-1,3,14-triol.

引用

页码：201 / 208

页数：8

共 33 条

[1]

Antiproliferative Action of Xylopia aethiopica Fruit Extract on Human Cervical Cancer Cells [J].