Overexpression of miR-483-5p/3p cooperate to inhibit mouse liver fibrosis by suppressing the TGF-β stimulated HSCs in transgenic mice

被引：84

作者：

Li, Fuyuan ^{[1
]}

Ma, Ning ^{[1
,2
]}

Zhao, Ruiqi ^{[1
]}

Wu, Guodong ^{[1
]}

Zhang, Yanfen ^{[3
]}

Qiao, Yu ^{[1
]}

Han, Dong ^{[1
]}

Xu, Ya ^{[1
]}

Xiang, Ying ^{[1
,4
]}

Yan, Bingzhu ^{[5
]}

Jin, Jianfeng ^{[1
]}

Lv, Guixiang ^{[1
]}

Wang, Lei ^{[1
]}

Xu, Changqing ^{[6
]}

Gao, Xu ^{[1
,2
,7
,8
]}

Luo, Shanshun ^{[9
]}

机构：

[1] Harbin Med Univ, Dept Biochem & Mol Biol, Harbin, Peoples R China

[2] Translat Med Ctr Northern China, Harbin, Peoples R China

[3] Harbin Med Univ, Affiliated Hosp, Dept Lab Diag, Harbin, Peoples R China

[4] Yangtze Univ, Sch Med, Dept Cell Biol, Jinzhou, Peoples R China

[5] Harbin Med Univ, Affiliated Hosp 2, Dept Infect Dis, Harbin, Peoples R China

[6] Harbin Med Univ, Dept Pathophysiol, Harbin, Peoples R China

[7] Heilongjiang Med Sci Acad, Basic Med Inst, Harbin, Peoples R China

[8] Harbin Med Univ, Minist Educ, Key Lab Cardiovasc Med Res, Harbin, Peoples R China

[9] Harbin Med Univ, Affiliated Hosp 1, Dept Gerontol, Harbin, Peoples R China

来源：

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2014年 / 18卷 / 06期

关键词：

liver fibrosis; microRNA; HSCs; transgenic mice; HEPATOCELLULAR-CARCINOMA; ENDOTHELIAL-CELLS; GROWTH-FACTORS; IN-VITRO; MICRORNAS; IDENTIFICATION; EXPRESSION; REVEALS; MIR-122; MODEL;

D O I：

10.1111/jcmm.12293

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

The transition from liver fibrosis to hepatocellular carcinoma (HCC) has been suggested to be a continuous and developmental pathological process. MicroRNAs (miRNAs) are recently discovered molecules that regulate the expression of genes involved in liver disease. Many reports demonstrate that miR-483-5p and miR-483-3p, which originate from miR-483, are up-regulated in HCC, and their oncogenic targets have been identified. However, recent studies have suggested that miR-483-5p/3p is partially down-regulated in HCC samples and is down-regulated in rat liver fibrosis. Therefore, the aberrant expression and function of miR-483 in liver fibrosis remains elusive. In this study, we demonstrate that overexpression of miR-483 in vivo inhibits mouse liver fibrosis induced by CCl4. We demonstrate that miR-483-5p/3p acts together to target two pro-fibrosis factors, platelet-derived growth factor- and tissue inhibitor of metalloproteinase 2, which suppress the activation of hepatic stellate cells (HSC) LX-2. Our work identifies the pathway that regulates liver fibrosis by inhibiting the activation of HSCs.

引用

页码：966 / 974

页数：9

共 40 条

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