Surface plasmon resonance imaging system with Mach-Zehnder phase-shift interferometry for DNA micro-array hybridization

被引：6

作者：

Hsiu, FM ^{[1
]}

Chen, SJ ^{[1
]}

Tsai, CH ^{[1
]}

Tsou, CY ^{[1
]}

Su, YD ^{[1
]}

Lin, GY ^{[1
]}

Huang, KT ^{[1
]}

Chyou, JJ ^{[1
]}

Ku, WC ^{[1
]}

Chiu, SK ^{[1
]}

Tzeng, CM ^{[1
]}

机构：

[1] Natl Cent Univ, Dept Mech Engn, Chungli 320, Taiwan

来源：

POLARIZATION MEASUREMENT, ANALYSIS, AND APPLICATIONS V | 2002年 / 4819卷

关键词：

SPR imaging; biomolecular interaction analysis; DNA hybridization; phase-shifting interferometry; Mach-Zehnder interferometer;

D O I：

10.1117/12.450922

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Surface plasmon resonance (SPR) imaging system is presented as a novel technique based on modified Mach-Zehnder phase-shifting interferometry (PSI) for biomolecular interaction analysis (BIA), which measures the spatial phase variation of a resonantly reflected light in biomolecular interaction. In this technique, the micro-array SPR biosensors with over a thousand probe NDA spots can be detected simultaneously. Owing to the feasible and swift measurements, the micro-array SPR biosensors can be extensively applied to the nonspecific adsorption of protein, the membrane/protein interactions, and DNA hybridization. The detection sensitivity of the SPR PSI imaging system is improved to about 1 pg/mm(2) for each spot over the conventional SPR imaging systems. The SPR PSI imaging system and its SPR sensors have been successfully used to observe slightly index change in consequence of argon gas flow through the nitrogen in real time, with high sensitivity, and at high-throughout screening rates.

引用

页码：167 / 174

页数：8

共 14 条

[1] Real-time measurement of nucleic-acid hybridization using evanescent-wave sensors: steps towards the genosensor [J].