Deoxycholic acid formation in gnotobiotic mice associated with human intestinal bacteria

被引:86
作者
Narushima, Seiko
Itoh, Kikuji
Miyamoto, Yukiko
Park, Sang-Hee
Nagata, Keiko
Kuruma, Kazuo
Uchida, Kiyohisa
机构
[1] Univ Tokyo, Lab Vet Publ Hlth, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
[2] Shionogi Res Labs, Toyonaka, Osaka 5610825, Japan
[3] Tottori Univ, Sch Med, Dept Pathophysiol & Therapeut Sci, Yonago, Tottori 6838503, Japan
关键词
D O I
10.1007/s11745-006-5038-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In humans and animals, intestinal flora is indispensable for bile acid transformation. The goal of our study was to establish gnotobiotic mice with intestinal bacteria of human origin in order to examine the role of intestinal bacteria in the transformation of bile acids in vivo using the technique of gnotobiology. Eight strains of bile acid-deconjugating bacteria were isolated from ex-germ-free mice inoculated with a human fecal dilution of 10(-6), and five strains of 7 alpha-dehydroxylating bacteria were isolated from the intestine of limited human flora mice inoculated only with clostridia. The results of biochemical tests and 16S rDNA sequence analysis showed that seven out of eight bile acid-deconjugating strains belong to a bacteroicles cluster (Bacteroides vulgatus, B. distasonis, and B. uniformis), and one strain had high similarity with Bilophila wadsworthia. All five strains that converted cholic acid to deoxycholic acid had greatest similarity with Clostridium hylemonae. A combination of 10 isolated strains converted taurocholic acid into deoxycholic acid both in vitro and in the mouse intestine. These results indicate that the predominant bacteria, mainly Bacteroides, in human feces comprise one of the main bacterial groups for the deconjugation of bile acids, and clostridia may play an important role in 7(alpha-dehydroxylation of free-form primary bile acids in the intestine although these strains are not predominant. The gnotobiotic mouse with bacteria of human origin could be a useful model in studies of bile acid metabolism by human intestinal bacteria in vivo.
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页码:835 / 843
页数:9
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