Does diphenylene iodonium chloride have any effect on the O2--generating step of plant peroxidases?

被引：11

作者：

Barceló, AR ^{[1
]}

Ferrer, MA ^{[1
]}

机构：

[1] Univ Murcia, Dept Plant Biol Plant Physiol, E-30100 Murcia, Spain

来源：

FEBS LETTERS | 1999年 / 462卷 / 03期

关键词：

compound III stability; peroxidase; diphenylene iodonium chloride; O-2(center dot-)-generating activity;

D O I：

10.1016/S0014-5793(99)01542-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The O-2(.-)-generating step of plant peroxidases during their catalytic cycle is represented by the decay of compound III (CoIII) into ferriperoxidase, which most likely involves the dissociation of a ferric-O-2(.-)- complex to yield the ferric form of the enzyme and O-2(.-), Diphenylene iodonium chloride (DPI), at 50-100 mu M, does not significantly enhance the stability of Com of peroxidase, as judged by the values of k(decay) and therefore, DPI appears to have no effect on the O-2(.-)-generating step of plant peroxidases. From these results, it is concluded that caution should be exercized when considering peroxidase as a possible enzyme target of O-2(.-)-mediated plant physiological processes sensitive to DPI inhibition. (C) 1999 Federation of European Biochemical Societies.

引用

页码：254 / 256

页数：3

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