A molecular marker for evaluating the pathogenic potential of foodborne Listeria monocytogenes

被引:199
作者
Jacquet, C
Doumith, M
Gordon, JI
Martin, PMV
Cossart, P
Lecuit, M
机构
[1] Inst Pasteur, Unite Interact Bacteries Cellules, INSERM, U604, F-75724 Paris 15, France
[2] Inst Pasteur, Lab Listeria, Ctr Natl Reference Listeria, WHO,Collaborating Ctr Foodborne Listeriosis, F-75724 Paris 15, France
[3] Washington Univ, Sch Med, Dept Mol Biol & Pharmacol, St Louis, MO USA
关键词
D O I
10.1086/420853
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background. Internalin mediates entry of Listeria monocytogenes into some human cultured cell lines and crossing of the intestinal barrier in transgenic mice expressing its receptor, human E-cadherin, in enterocytes. The relevance of these findings for humans is challenged by the observation that some L. monocytogenes isolates express a truncated nonfunctional form of internalin. Methods. We investigated expression of internalin by use of immunoblot assay in 300 clinical strains obtained in France in a single year and a representative set of 150 strains obtained from food products during the same period. Results. Clinical strains expressed full-length internalin far more frequently (288/300 strains [96%]) than did strains recovered from food products (98/150 strains [65%]; odds ratio, 12.73; 95% confidence interval, 6.27-26.34; P < 1 X 10(-7)). All 61 strains (100%) from pregnancy-related cases, 55 (98%) of 56 strains from patients with central nervous system infections, and 151 (93%) of 162 strains from patients with bacteremia expressed full-length internalin. All 110 strains belonging to serovar 4b, the most frequently implicated serovar in human listeriosis, expressed full-length internalin. Conclusions. This study demonstrates the critical role of internalin in the pathogenesis of human listeriosis. It provides a molecular explanation for the predominance of serovar 4b among clinical strains and supports the usefulness of studying the expression of internalin as a marker of virulence in humans.
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页码:2094 / 2100
页数:7
相关论文
共 41 条
[1]   An outbreak of febrile gastroenteritis associated with corn contaminated by Listeria monocytogenes. [J].
Aureli, P ;
Fiorucci, GC ;
Caroli, D ;
Marchiaro, G ;
Novara, O ;
Leone, L ;
Salmaso, S .
NEW ENGLAND JOURNAL OF MEDICINE, 2000, 342 (17) :1236-1241
[2]   Variation in the infectivity of Listeria monocytogenes isolates following intragastric inoculation of mice [J].
Barbour, AH ;
Rampling, A ;
Hormaeche, CE .
INFECTION AND IMMUNITY, 2001, 69 (07) :4657-4660
[3]   API LISTERIA, A NEW AND PROMISING ONE-DAY SYSTEM TO IDENTIFY LISTERIA ISOLATES [J].
BILLE, J ;
CATIMEL, B ;
BANNERMAN, E ;
JACQUET, C ;
YERSIN, MN ;
CANIAUX, I ;
MONGET, D ;
ROCOURT, J .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (06) :1857-1860
[4]   PULSED-FIELD FINGERPRINTING OF LISTERIAE - IDENTIFICATION OF GENOMIC DIVISIONS FOR LISTERIA-MONOCYTOGENES AND THEIR CORRELATION WITH SEROVAR [J].
BROSCH, R ;
CHEN, JC ;
LUCHANSKY, JB .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (07) :2584-2592
[5]  
Buzby J C, 1997, World Health Stat Q, V50, P57
[6]  
*CDCP, 2002, JAMA-J AM MED ASSOC, V288, P2260
[7]   Invasion of mammalian cells by Listeria monocytogenes:: functional mimicry to subvert cellular functions [J].
Cossart, P ;
Pizarro-Cerdá, J ;
Lecuit, M .
TRENDS IN CELL BIOLOGY, 2003, 13 (01) :23-31
[8]   Interactions of Listeria monocytogenes with mammalian cells during entry and actin-based movement:: bacterial factors, cellular ligands and signaling [J].
Cossart, P ;
Lecuit, M .
EMBO JOURNAL, 1998, 17 (14) :3797-3806
[9]   Ability of the Listeria monocytogenes strain Scott A to cause systemic infection in mice infected by the intragastric route [J].
Czuprynski, CJ ;
Faith, NG ;
Steinberg, H .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (06) :2893-2900
[10]   An outbreak of gastroenteritis and fever due to Listeria monocytogenes in milk [J].
Dalton, CB ;
Austin, CC ;
Sobel, J ;
Hayes, PS ;
Bibb, WF ;
Graves, LM ;
Swaminathan, B ;
Proctor, ME ;
Griffin, PM .
NEW ENGLAND JOURNAL OF MEDICINE, 1997, 336 (02) :100-105