Linkage map organization of expressed sequence tags and sequence tagged sites in the mosquito, Aedes aegypti

被引：53

作者：

Severson, DW ^{[1
]}

Meece, JK ^{[1
]}

Lovin, DD ^{[1
]}

Saha, G ^{[1
]}

Morlais, I ^{[1
]}

机构：

[1] Univ Notre Dame, Dept Biol Sci, Ctr Trop Dis Res & Training, Notre Dame, IN 46556 USA

来源：

INSECT MOLECULAR BIOLOGY | 2002年 / 11卷 / 04期

关键词：

EST; genomics; RFLP; SNP; SSCP;

D O I：

10.1046/j.1365-2583.2002.00347.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A composite genetic linkage map for the yellow fever mosquito Aedes aegypti was constructed based on restriction fragment length polymorphism (RFLP), single nucleotide polymorphism (SNP) and single strand conformation polymorphism (SSCP) markers. The map consists of 146 marker loci distributed across 205 cM, and includes several morphological mutant marker loci. Most of the genetic markers are derived from random cDNAs or Ale. aegypti genes of known function. A number of markers are derived from random genomic DNAs, including several cloned RAPD-PCR fragments, and also several cDNAs from Drosophila melanogaster. Most of the random cDNAs (80.2%) have high BLASTX sequence identities to known genes, with the majority of matches to genes from D. melanogaster. Access to sequence data for all markers will facilitate their continued development for use in high-throughput SNP marker analyses and also provides additional physical anchor points for an anticipated genome sequencing effort.

引用

页码：371 / 378

页数：8

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