Characterization of genes encoding ABA 8′-hydroxylase in ethylene-induced stem growth of deepwater rice (Oryza sativa L.)

被引：75

作者：

Yang, Seung-Hwan

Choi, Dongsu ^{[1
]}

机构：

[1] Kunsan Natl Univ, Sch Sci & Technol, Gunsan 573701, South Korea

[2] Michigan State Univ, Dept Energy, Plant Res Lab, E Lansing, MI 48824 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 350卷 / 03期

基金：

美国国家科学基金会;

关键词：

ABA 8'-hydroxylase abscisic acid; deepwater rice; ethylene; phaseic acid; stem elongation; (+)-ABSCISIC ACID 8'-HYDROXYLASE; ABSCISIC-ACID; INTERNODAL ELONGATION; EXPRESSION; GIBBERELLIN; PLANT; METABOLISM;

D O I：

10.1016/j.bbrc.2006.09.098

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ethylene and submergence enhance stem elongation of deepwater rice, at least in part, by reducing in the internode the endogenous abscisic acid (ABA) content and increasing the level of gibberellin A(1) (GA(1)). We cloned and characterized the CYP707A5 and CYP707A6 genes, which encode putative ABA 8'-hydroxylase, the enzyme that catalyzes the oxidation of ABA. Expression of CYP707A5 was upregulated significantly by ethylene treatment, whereas that of CYP707A6 was not altered. Recombinant proteins from both genes expressed in yeast cells showed activity of ABA 8'-hydroxylase. This finding indicates that CYP707A5 may play a role in ABA catabolism during submergence- or ethylene-induced stem elongation in deepwater rice. Taken together, these results provide links between the molecular mechanisms and physiological phenomena of submergence- and ethylene-induced stem elongation in deepwater rice. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：685 / 690

页数：6

共 22 条

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