Increased Oral Fibroblast Lifespan Is Telomerase-independent

被引：25

作者：

Enoch, S. ^{[1
]}

Wall, I. ^{[1
]}

Peake, M. ^{[1
]}

Davies, L. ^{[1
]}

Farrier, J. ^{[1
]}

Giles, P. ^{[2
]}

Baird, D. ^{[2
]}

Kipling, D. ^{[2
]}

Price, P. ^{[3
]}

Moseley, R. ^{[1
]}

Thomas, D. ^{[1
]}

Stephens, P. ^{[1
]}

机构：

[1] Cardiff Univ, Wound Biol Grp, Cardiff Inst Tissue Engn & Repair, Sch Dent, Cardiff CF14 4XY, S Glam, Wales

[2] Cardiff Univ, Dept Pathol, Sch Med, Cardiff CF14 4XY, S Glam, Wales

[3] Cardiff Univ, Sch Med, Wound Healing Res Unit, Cardiff CF14 4XY, S Glam, Wales

来源：

JOURNAL OF DENTAL RESEARCH | 2009年 / 88卷 / 10期

关键词：

telomeres; fibroblast; aging; oral mucosa; EXTRACELLULAR-MATRIX REORGANIZATION; PHENOTYPIC DIFFERENCES; HUMAN-CELLS; SKIN; MUCOSAL; HETEROGENEITY; SENESCENCE; CULTURE; AGE;

D O I：

10.1177/0022034509342979

中图分类号：

R78 [口腔科学];

学科分类号：

100302 [口腔临床医学];

摘要：

Oral mucosal wound-healing is characterized by rapid re-epithelialization and remodeling, with minimal scar formation. This may be attributed to the distinct phenotypic characteristics of the resident fibroblasts. To test this hypothesis, we investigated patient-matched oral mucosal and skin fibroblasts. Compared with skin fibroblasts, oral mucosal fibroblasts had longer proliferative lifespans, underwent more population doublings, and experienced senescence later, which was directly related to longer telomere lengths within oral mucosal fibroblasts. The presence of these longer telomeres was independent of telomerase expression, since both oral oral mucosal fibroblasts and skin fibroblasts were negative for active telomerase, as assessed according to the Telomeric Repeat Amplification Protocol. This study has demonstrated that, compared with skin fibroblasts, oral mucosal fibroblasts are 'younger', with a more embryonic/fetal-like phenotype that may provide a notable advantage for their ability to repair wounds in a scarless fashion.

引用

页码：916 / 921

页数：6

共 35 条

[1]

An investigation of preferential fibroblast wound repopulation using a novel in vitro wound model [J].