Molecular profiling to identify molecular mechanism in esophageal cancer with familial clustering

被引：51

作者：

Chattopadhyay, Indranil ^{[1
]}

Phukan, Rupkumar ^{[3
]}

Singh, Avninder ^{[1
]}

Vasudevan, Madavan ^{[4
]}

Purkayastha, Joydeep ^{[2
]}

Hewitt, Stepen ^{[5
]}

Kataki, Amal ^{[2
]}

Mahanta, Jagadish ^{[3
]}

Kapur, Sujala ^{[1
]}

Saxena, Sunita ^{[1
]}

机构：

[1] Inst Pathol, New Delhi 110029, India

[2] Dr B Borooah Canc Inst, Gauhati 781016, Assam, India

[3] Reg Med Res Ctr, Dibrugarh 786001, Assam, India

[4] Genotyp Technol P Ltd, Bangalore 560001, Karnataka, India

[5] NCI, Pathol Lab, Bethesda, MD 20892 USA

来源：

ONCOLOGY REPORTS | 2009年 / 21卷 / 05期

关键词：

expression profile; familial esophageal cancer; microarray; ENDOTHELIAL GROWTH-FACTOR; EXPRESSION; CELLS; TUMOR; ANGIOGENESIS; ARGINASE;

D O I：

10.3892/or_00000333

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

To identify the genes and molecular functional pathways involved in esophageal cancer, we analyzed the gene expression profile of esophageal tumor tissue from patients having family history of esophageal cancer by cDNA microarray. Three hundred and fifty differentially expressed genes (26 up-regulated and 324 down-regulated) were identified. Genes involved in humoral immune response (PF4), extracellular matrix organization (COL4A4), metabolism of xenobiotics (EPHX1), TGF-beta signaling (SMAD1) and calcium signaling pathways (VDAC1) were down-regulated and genes involved in regulation of actin cytoskeleton (WASL), neuroactive ligand receptor interaction (GRM3), Toll-like receptor (CD14), B-cell receptor (IFITM1) and insulin signaling pathways (FOXO1A) were up-regulated. Validation of differential expression of subset of genes by QRT-PCR and tissue microarray in familial and non-familial cases showed no significant difference in expression of these genes in two groups suggesting familial clustering occurs as result of sharing of common environmental factors. Gene expression profiling of clinical specimens from well characterized populations that have familial clustering of cancer identified molecular mechanism associated with progression of esophageal cancer.

引用

页码：1135 / 1146

页数：12

共 28 条

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Hyaluronan-CD44 interaction with neural Wiskott-Aldrich syndrome protein (N-WASP) promotes actin polymerization and ErbB2 activation leading to β-catenin nuclear translocation, transcriptional up-regulation, and cell migration in ovarian tumor cells [J].