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Glucocorticoid-Induced Leucine Zipper Is Protective in Th1-Mediated Models of Colitis
被引:102
作者:
Cannarile, Lorenza
[2
]
Cuzzocrea, Salvatore
[1
,3
]
Santucci, Luca
[4
]
Agostini, Massimiliano
[2
]
Mazzon, Emanuela
[3
]
Esposito, Emanuela
[3
,5
]
Muia, Carmelo
[1
]
Coppo, Maddalena
[2
]
Di Paola, Rosanna
[3
]
Riccardi, Carlo
[2
]
机构:
[1] Univ Messina, Sch Med, Dept Clin & Expt Med & Pharmacol, Messina, Italy
[2] Univ Perugia, Dept Clin & Expt Med, Sect Pharmacol & Polo Sci & Didatt Terni, I-06100 Perugia, Italy
[3] IRCCS Ctr Neurolesi Bonino Pulejo, Messina, Italy
[4] Azienda Osped Perugia, Perugia, Italy
[5] Univ Naples Federico II, Dept Expt Pharmacol, Naples, Italy
关键词:
NF-KAPPA-B;
INFLAMMATORY-BOWEL-DISEASE;
T-CELL ACTIVATION;
APOPTOSIS;
IMMUNE;
GENE;
TRANSCRIPTION;
INHIBITION;
EXPRESSION;
MICE;
D O I:
10.1053/j.gastro.2008.09.024
中图分类号:
R57 [消化系及腹部疾病];
学科分类号:
摘要:
Background & Aims: Inflammatory bowel diseases are relatively common diseases of the gastrointestinal tract. The relative therapeutic efficacy of glucocorticoids used in inflammatory bowel diseases resides in part in their capability to inhibit activity of nuclear factor kappa B (NF-kappa B), a transcription factor central to the inflammatory process, and the consequent production of T-helper 1 (Th1)-type cytokines. Previous studies indicate that increased expression in transgenic mice of glucocorticoid-induced leucine zipper (GILZ), a gene rapidly induced by glucocorticoids, inhibits NF-kappa B and Th1 activity. Methods: We performed experiments with the aim to test the susceptibility of GILZ transgenic (GILZ-TG) mice to dinitrobenzene sulfonic acid-induced colitis. Results: Consistent with a decreased Th1 response, GILZ-TG mice were less susceptible to colitis induction as compared with wild-type littermates, while they were more susceptible to Th2-mediated colitis. The inhibition was comparable to that obtained with dexamethasone treatment. Moreover, diminished intestinal tissue damage, associated with inhibition of NF-kappa B nuclear translocation, interferon-gamma, tumor necrosis factor-alpha, and interleukin-1 production in CD4(+) T lymphocytes of the lamina propria, was evident in GILZ-TG as compared with wild-type mice. In addition, inhibition of colitis development was also evident when GILZ fusion protein was delivered in vivo in dinitrobenzene sulfonic acid-treated WT animals as well as in interleukin-10 knockout mice. Conclusions: Together these results demonstrate that GILZ mimics the effects of glucocorticoids, suggesting a contribution of this protein to the and-inflammatory activity of glucocorticoids in Th1-induced colitis.
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页码:530 / 541
页数:12
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