Transcriptome and Proteome Analysis of Osteocytes Treated with Nitrogen-Containing Bisphosphonates

被引：17

作者：

Bivi, Nicoletta ^{[2
]}

Bereszczak, Jessica Z. ^{[1
]}

Romanello, Milena ^{[2
]}

Zeef, Leo A. H. ^{[3
]}

Delneri, Daniela ^{[3
]}

Quadrifoglio, Franco ^{[2
]}

Moro, Luigi ^{[4
]}

Brancia, Francesco L. ^{[1
]}

Tell, Gianluca ^{[2
]}

机构：

[1] Shimadzu Res Lab Europe, Manchester, Lancs, England

[2] Univ Udine, Dept Biomed Sci & Technol, I-33100 Udine, Italy

[3] Univ Manchester, Dept Life Sci, Manchester M13 9PL, Lancs, England

[4] Ctr Study Metab Bone Dis, I-34170 Gorizia, Italy

来源：

JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 03期

关键词：

bisphosphonates; osteocytes; differential proteomics; gene expression; GLaD; isotopic labelling; LABELING DERIVATIZATION GLAD; RENAL EPITHELIAL-CELLS; BONE-RESORPTION; PROTON PUMP; ACTIVATION; OSTEOCLASTS; OSTEOBLASTS; MECHANISMS; EXPRESSION; APOPTOSIS;

D O I：

10.1021/pr8005606

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

We combined high-throughput screening of differential mRNAs with mass spectrometric characterization of proteins obtained from osteocytes untreated and treated with Risedronate. Microarray analysis revealed, upon treatment, a marked upregulation of messengers encoding zinc-proteins. MS analysis identified 84 proteins in the osteocytes proteome map. Risedronate affected the expression of 10 proteins, associated with cytoskeleton, stress-response and metabolism. Data validated using gel imaging in combination with the GLaD post digestion isotopic labeling method provide the molecular basis for understanding the role of bisphosphonates as antiapoptotic drugs for osteocytes.

引用

页码：1131 / 1142

页数：12

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