A novel approach to the solubility measurement of protein crystals by two-beam interferometry

被引：83

作者：

Sazaki, G

Kurihara, K

Nakada, T

Miyashita, S

Komatsu, H

机构：

[1] Institute for Materials Research, Tohoku University, Sendai 980-77, Katahira 2-1-1, Aoba-ku

来源：

JOURNAL OF CRYSTAL GROWTH | 1996年 / 169卷 / 02期

关键词：

interferometers; metastable phases; solubility; segregation; and mixing; phase separation;

D O I：

10.1016/S0022-0248(96)00442-3

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

A novel technique was developed for the rapid solubility measurement of protein crystals (lysozyme) by two-beam interferometry. The merits of this technique are: (1) time saving (less than 2 h to obtain one experimental point), (2) the small amount of the sample (similar to 66 mu l), (3) high accuracy, (4) applicable to the solubility measurement of a metastable phase and (5) insensitive to impurities. The limitations of the present technique are also discussed.

引用

收藏

页码：355 / 360

页数：6

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