Colorimetric detection of hepatitis E virus based on reverse transcription loop mediated isothermal amplification (RT-LAMP) assay

被引：18

作者：

Chen Qiuhua

Yuan Lu ^{[1
]}

Wan Jun ^{[2
]}

Chen Yanlin

Du Chaoyang

机构：

[1] 452 Hosp Chengdu, Peoples Liberat Army, Chengdu 610021, Sichuan, Peoples R China

[2] Chinese PLA 458th Hosp, Guangzhou 510602, Guangdong, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2014年 / 197卷

关键词：

Hepatitis E virus; Calorimetric detection; Loop mediated isothermal amplification;

D O I：

10.1016/j.jviromet.2013.11.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Hepatitis E virus (HEV) infection is a common communicable disease in the third world countries. The early diagnosis of HEV is important for the prognosis and prevention of the spread of the disease. Conventional methods for HEV detection need expensive equipment and cannot meet the needs of point of care (POC) use. In the present study, a gold nanoparticle (AuNP) based RT-LAMP assay was developed for colorimetric detection of HEV. As low as 10(1) copies of HEV RNA were detected visually. With reduced handling and less equipment requirement, this assay provided a simple and rapid alternative to other available methods. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：29 / 33

页数：5

共 10 条

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