Aqueous chromatography system using pH- and temperature-responsive stationary phase with ion-exchange groups

被引:52
作者
Ayano, Eri
Nambu, Kyoko
Sakamoto, Chikako
Kanazawa, Hideko
Kikuchi, Akihiko
Okano, Teruo
机构
[1] Kyoritsu Univ Pharm, Dept Phys Pharmaceut Chem, Minato Ku, Tokyo 1058512, Japan
[2] Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Shinjyuku Ku, Tokyo 1628666, Japan
关键词
poly(N-isopropylacrylamide); HPLC; pH- and temperature-responsive chromatography; NSAIDs; ibuprofen; ketoprofen; naproxen;
D O I
10.1016/j.chroma.2006.01.068
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We report on the development of a novel analytical HPLC technique of nonsteroidal anti-inflammatory drugs (NSAIDs), such as ibuprofen, ketoprofen and naproxen, with an isocratic aqueous mobile phase. In this study, we designed a new pH- and temperature-responsive copolymer of N-isopropylacrylamide (NIPAAm), butyl methacrylate (BMA) and N,N-dimethylaminopropylacrylamide (DMAPAAm). The copolymer was modified with cross-linked poly(NIPAAm-co-BMA-co-DMAPAAm) (IBD) hydrogel on to aminopropyl silica beads, and the products were evaluated as HPLC packing materials for an ion-exchange- and temperature-responsive chromatography. The property of the surface of the stationary phase was altered from hydrophilic to hydrophobic, and from charged to non-charged by changes in the temperature and pH. In addition, it is possible that ion-exchange groups can appear or be hidden on the polymer chain surface by temperature changes. The interactions of NSAIDs with this stationary phase were controlled by the temperature and the pH with a constant aqueous mobile phase. PH- and temperature-responsive chromatography is expected to be useful for the separation of pharmaceuticals and biomolecules. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:58 / 65
页数:8
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