Anti-peptide antibodies sensitive to the 'active' state of the beta(2)-adrenergic receptor

被引：33

作者：

Mijares, A

Lebesgue, D

Argibay, J

Hoebeke, J

机构：

[1] UFR SCI PHARMACEUT,FAC SCI,CNRS,UMR 6542,F-37200 TOURS,FRANCE

[2] UFR SCI PHARMACEUT,EQUIPE IMMUNOL RECEPTEURS,INSERM,CJF 9309,F-37200 TOURS,FRANCE

来源：

FEBS LETTERS | 1996年 / 399卷 / 1-2期

关键词：

G protein-coupled receptor; conformer; beta(2)-adrenergic receptor; antibody; peptide; calcium current;

D O I：

10.1016/S0014-5793(96)01321-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Antibodies directed against a peptide corresponding to the second loop of the human beta(2)-adrenergic receptor were induced in rabbits by immunisation with the free peptide in complete Freund's adjuvant. The resulting antibodies were affinity-purified and shown to be monospecific for the target receptor. They were able to stimulate the L-type Ca2+ channels in whole-cell patch-clamp experiments on isolated adult guineapig cardiomyocytes. This effect was similar to that obtained by the specific beta(2)-adrenergic agonist zinterol. The antibody effects could be blocked with the specific beta(2)-adrenergic inverse agonist ICI118,551 but not with the neutral antagonist alprenolol. These results suggest that the antibodies recognise the active conformer of the beta(2)-adrenergic receptor.

引用

收藏

页码：188 / 191

页数：4

相关论文

共 32 条

[1]

Abd Alla S., 1996, J BIOL CHEM, V271, P1748

[2] (-)-S-[H-3]CGP-12177 AND ITS USE TO DETERMINE THE RATE CONSTANTS OF UNLABELED BETA-ADRENERGIC ANTAGONISTS [J].

AFFOLTER, H ;

HERTEL, C ;

JAEGGI, K ;

PORTENIER, M ;

STAEHELIN, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (03) :925-929

[3] ISOPROTERENOL RESPONSE FOLLOWING TRANSFECTION OF THE MOUSE BETA-2-ADRENERGIC RECEPTOR GENE INTO Y1-CELLS [J].

ALLEN, JM ;

BAETGE, EE ;

ABRASS, IB ;

PALMITER, RD .

EMBO JOURNAL, 1988, 7 (01) :133-138

[4] RESPONSE OF FAILING CANINE AND HUMAN HEART-CELLS TO BETA(2)-ADRENERGIC STIMULATION [J].

ALTSCHULD, RA ;

STARLING, RC ;

HAMLIN, RL ;

BILLMAN, GE ;

HENSLEY, J ;

CASTILLO, L ;

FERTEL, RH ;

HOHL, CM ;

ROBITAILLE, PML ;

JONES, LR ;

XIAO, RP ;

LAKATTA, EG .

CIRCULATION, 1995, 92 (06) :1612-1618

[5] PHYSIOLOGICAL-EFFECTS OF INVERSE AGONISTS IN TRANSGENIC MICE WITH MYOCARDIAL OVEREXPRESSION OF THE BETA(2)-ADRENOCEPTOR [J].

BOND, RA ;

LEFF, P ;

JOHNSON, TD ;

MILANO, CA ;

ROCKMAN, HA ;

MCMINN, TR ;

APPARSUNDARAM, S ;

HYEK, MF ;

KENAKIN, TP ;

ALLEN, LF ;

LEFKOWITZ, RJ .

NATURE, 1995, 374 (6519) :272-276

[6]

CHIDIAC P, 1994, MOL PHARMACOL, V45, P490

[7]

COSTA T, 1992, MOL PHARMACOL, V41, P549

[8] CLONING OF THE GENE AND CDNA FOR MAMMALIAN BETA-ADRENERGIC-RECEPTOR AND HOMOLOGY WITH RHODOPSIN [J].

DIXON, RAF ;

KOBILKA, BK ;

STRADER, DJ ;

BENOVIC, JL ;

DOHLMAN, HG ;

FRIELLE, T ;

BOLANOWSKI, MA ;

BENNETT, CD ;

RANDS, E ;

DIEHL, RE ;

MUMFORD, RA ;

SLATER, EE ;

SIGAL, IS ;

CARON, MG ;

LEFKOWITZ, RJ ;

STRADER, CD .

NATURE, 1986, 321 (6065) :75-79

[9] STRUCTURE OF THE GENE FOR HUMAN BETA-2-ADRENERGIC RECEPTOR - EXPRESSION AND PROMOTER CHARACTERIZATION [J].

EMORINE, LJ ;

MARULLO, S ;

DELAVIERKLUTCHKO, C ;

KAVERI, SV ;

DURIEUTRAUTMANN, O ;

STROSBERG, AD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (20) :6995-6999

[10] CLONING OF THE CDNA FOR THE HUMAN BETA-1-ADRENERGIC RECEPTOR [J].

FRIELLE, T ;

COLLINS, S ;

DANIEL, KW ;

CARON, MG ;

LEFKOWITZ, RJ ;

KOBILKA, BK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (22) :7920-7924

← 1 2 3 4 →