Sequence and expression of a novel member (LTBP-4) of the family of latent transforming growth factor-beta binding proteins

被引:64
作者
Giltay, R [1 ]
Kostka, G [1 ]
Timpl, R [1 ]
机构
[1] MAX PLANCK INST BIOCHEM,D-82152 MARTINSRIED,GERMANY
关键词
extracellular matrix; microfibrillar protein; TGF-beta; 1; binding; tissue expression;
D O I
10.1016/S0014-5793(97)00685-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Overlapping cDNA clones from human heart and melanoma libraries were used to establish the 1587-residue sequence of a novel protein (LTBP-4) belonging to the family of extracellular microfibrillar proteins which also bind transforming growth factor-beta. LTBP-4 consists of 20 EG modules, 17 of them with a consensus sequence for calcium binding, 4 TB modules with 8 cysteines and several proline-rich regions, Northern blots demonstrated a single 5 kb mRNA which is highly expressed in heart but also present in skeletal muscle, pancreas, placenta and lung. The modular structure predicts that LTBP-4 should be a microfibrillar protein,which probably also binds TGF-beta. (C) 1997 Federation of European Biochemical Societies.
引用
收藏
页码:164 / 168
页数:5
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