Autoantigen arrays for multiplex analysis of antibody isotypes

被引：17

作者：

Graham, Kareem L. ^{[1
]}

Vaysberg, Maria ^{[1
]}

Kuo, Annie ^{[1
]}

Utz, Paul J. ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Med, Div Immunol & Rheumatol, Stanford, CA 94305 USA

来源：

PROTEOMICS | 2006年 / 6卷 / 21期

关键词：

autoantibody profiling; autoantigen; isotype; microarray;

D O I：

10.1002/pmic.200600345

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe here a microarray-based method for multiplexed, antigen-specific assessment of immunoglobulin (Ig) subclasses. We used 1152-feature arrays composed of 140 antigens or antigen fragments to detect isotype-specific mAb, to quantitatively monitor changes in isotype mAb concentration, and to profile antigen-specific antibody isotype production in a murine model of autoimmunity. This platform can be easily adapted to a variety of applications, and has the potential to elucidate mechanisms that govern development and evolution of antibody responses in in vivo and in vitro systems.

引用

页码：5720 / 5724

页数：5

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