One-Step Synthesis of Folic Acid Protected Gold Nanoparticles and Their Receptor-Mediated Intracellular Uptake

被引：79

作者：

Li, Gaiping ^{[1
,2
]}

Li, Dan ^{[1
,2
]}

Zhang, Lixue ^{[1
,2
]}

Zhai, Junfeng ^{[1
,2
]}

Wang, Erkang ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

[2] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2009年 / 15卷 / 38期

基金：

中国国家自然科学基金;

关键词：

competitive inhibition; folic acid; gold; nanoparticles; reporter-mediated uptake; SURFACE MODIFICATION; CELLULAR UPTAKE; CELLS; NANORODS; DELIVERY; THERAPY; CITRATE; AGENTS; FUNGUS; SIZE;

D O I：

10.1002/chem.200900914

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

We report here a facile method to obtain folic acid (FA)-protected gold nanoparticles (Au NPs) by heating an aqueous solution of HAuCl4/FA in which FA acts as both the reducing and stabilizing agent. The successful formation of FA-protected Au NPs is demonstrated by UV/Vis spectroscopy, transmission electron microscopy (TEM), selected-area electron diffraction (SAED), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR). ne intracellular uptake of these nanoparticles is facilitated by HeLa cells overexpressing the folate reporter, which itself is significantly inhibited by free FA in a competitive assay as quantified by inductively coupled plasma mass spectroscopy (ICP-MS). This simple one-step approach affords a new perspective for creating functional nanomaterials, and the resulting biocompatible, functional Au NPs may find some prospective applications in various biomedical fields.

引用

页码：9868 / 9873

页数：6

共 44 条

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