Bacteriocins reduce Campylobacter colonization and alter gut morphology in turkey poults

被引:55
作者
Cole, K.
Farnell, M. B.
Donoghue, A. M.
Stern, N. J.
Svetoch, E. A.
Eruslanov, B. N.
Volodina, L. I.
Kovalev, Y. N.
Perelygin, V. V.
Mitsevich, E. V.
Mitsevich, I. P.
Levchuk, V. P.
Pokhilenko, V. D.
Borzenkov, V. N.
Svetoch, O. E.
Kudryavtseva, T. Y.
Reyes-Herrera, I.
Blore, P. J.
de los Santos, F. Solis
Donoghue, D. J. [1 ]
机构
[1] Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA
[2] USDA ARS, Poultry Prod & Prod Safety Res Unit, Fayetteville, AR 72701 USA
[3] State Res Ctr Appl Microbiol, Obolensk, Russia
[4] USDA ARS, Poultry Microbiol Safety Res UNit, Russell Res Ctr, Athens 30604, Greece
关键词
Campylobacter; ceca; bacteriocin; turkey; gastrointestinal tract;
D O I
10.1093/ps/85.9.1570
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Campylobacter is a leading cause of foodborne illness in the United States. Recent evidence has demonstrated that bacteriocins produced by Bacillus circulans and Paenibacillus polymyxa reduce cecal Campylobacter colonization in broiler chickens infected with Campylobacter jejuni. As Campylobacter coli is the most prevalent Campylobacter isolate recovered in turkeys, the objectives of the present study were to evaluate the efficacy of these bacteriocins against C. coli colonization and their influence on the gastrointestinal architecture of young turkeys. In 3 separate trials, a total of 135 day-of-hatch poults (n = 45/trial) were orally challenged on d 3 with approximately 10(6) cfu of a mixture of 3 C. coli isolates. Immediately before bacteriocin treatment (d 10), cecal Campylobacter concentrations averaged 1. 1 X 107 CfU/ g of cecal contents (n = 15/trial). On d 10 to 12 posthatch, 2 bacteriocin treatment groups were given free access to feed supplemented with purified, microencapsulated bacteriocins, whereas the positive control treatment group had access to untreated feed (n = 10/treatment group per trial). At the end of the 3-d dosing period, ceca and duodenal loops were collected for analysis. In each of the 3 separate trials, treatment with bacteriocin eliminated detectable ceca Campylobacter concentrations (detection limit, 1 x 10(2) cfu/g of cecal contents) vs. controls (1.0 x 10(6) cfu of Campylobacter/g of cecal contents). Duodenum crypt depth and goblet cell numbers were also reduced in turkeys treated with either bacteriocin vs. controls (P < 0.05). The dynamic reduction in crypt depth and goblet cell density in turkeys dosed with bacteriocin may provide clues to how bacteriocins inhibit enteric Campylobacter.
引用
收藏
页码:1570 / 1575
页数:6
相关论文
共 42 条
[1]   Morphometric analysis of the intestine of domestic quails (Coturnix coturnix japonica) treated with different levels of dietary calcium [J].
Aptekmann, KP ;
Artoni, SMB ;
Stefanini, MA ;
Orsi, MA .
ANATOMIA HISTOLOGIA EMBRYOLOGIA-JOURNAL OF VETERINARY MEDICINE SERIES C, 2001, 30 (05) :277-280
[2]   COLONIZATION OF GASTROINTESTINAL TRACTS OF CHICKS BY CAMPYLOBACTER-JEJUNI [J].
BEERY, JT ;
HUGDAHL, MB ;
DOYLE, MP .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (10) :2365-2370
[3]   Approach for selection of individual enteric bacteria for competitive exclusion in turkey poults [J].
Bielke, LR ;
Elwood, AL ;
Donoghue, DJ ;
Donoghue, AM ;
Newberry, LA ;
Neighbor, NK ;
Hargis, BM .
POULTRY SCIENCE, 2003, 82 (09) :1378-1382
[4]   Veterinary use of antimicrobials and emergence of resistance in zoonotic and sentinel bacteria in the EU [J].
Bywater, RJ .
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 2004, 51 (8-9) :361-363
[5]   Antagonistic activities of several bacteria on in vitro growth of 10 strains of Campylobacter jejuni/coli [J].
Chaveerach, P ;
Lipman, LJA ;
van Knapen, F .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2004, 90 (01) :43-50
[6]   ORIGIN, DIFFERENTIATION AND RENEWAL OF 4 MAIN EPITHELIAL-CELL TYPES IN MOUSE SMALL INTESTINE .5. UNITARIAN THEORY OF ORIGIN OF 4 EPITHELIAL-CELL TYPES [J].
CHENG, H ;
LEBLOND, CP .
AMERICAN JOURNAL OF ANATOMY, 1974, 141 (04) :537-&
[7]   Bacteriocins: safe, natural antimicrobials for food preservation [J].
Cleveland, J ;
Montville, TJ ;
Nes, IF ;
Chikindas, ML .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2001, 71 (01) :1-20
[8]   Effects of aeration and storage temperature on Campylobacter concentrations in poultry semen [J].
Cole, K ;
Donoghue, AM ;
Blore, PJ ;
Holliman, JS ;
Cox, NA ;
Musgrove, MT ;
Donoghue, DJ .
POULTRY SCIENCE, 2004, 83 (10) :1734-1738
[9]   TREATMENT OF COMMERCIAL BROILER-CHICKENS WITH A CHARACTERIZED CULTURE OF CECAL BACTERIA TO REDUCE SALMONELLAE COLONIZATION [J].
CORRIER, DE ;
NISBET, DJ ;
SCANLAN, CM ;
HOLLISTER, AG ;
CALDWELL, DJ ;
THOMAS, LA ;
HARGIS, BM ;
TOMKINS, T ;
DELOACH, JR .
POULTRY SCIENCE, 1995, 74 (07) :1093-1101
[10]   Poultry as a source of Campylobacter and related organisms [J].
Corry, JEL ;
Atabay, HI .
JOURNAL OF APPLIED MICROBIOLOGY, 2001, 90 :96S-114S