Extraction optimized by using response surface methodology, purification and preliminary characterization of polysaccharides from Hyriopsis cumingii

被引：198

作者：

Qiao, Deliang ^{[1
,2
]}

Hu, Bing ^{[1
]}

Gan, Dan ^{[1
]}

Sun, Yi ^{[1
]}

Ye, Hong ^{[1
]}

Zeng, Xiaoxiong ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Peoples R China

[2] W Anhui Univ, Dept Chem & Biol Sci, Luan 237012, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2009年 / 76卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Hyriopsis cumingii; Polysaccharides; Extraction; Optimization; Purification; Characterization; PECTIN EXTRACTION; PARAMETERS; COMPONENTS;

D O I：

10.1016/j.carbpol.2008.11.004

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Extraction, purification and preliminary characterization of polysaccharides from Hyriopsis cumingii (HCPS) were carried out. Firstly, the extracting parameters for HCPS extraction were optimized by using three-factor-three-level Box-Behnken design and response surface methodology based on the single-factor experiments. As results, the optimum conditions were extracting temperature 80 degrees C, extracting time 4.5 h and the ratio of extraction solvent (water) to raw material (v/w) 8. Under these conditions, the experimental yield of crude HCPS was 3.66%. Secondly, the crude HCPS was purified by DEAE-Cellulose 52 chromatography and Sephadex G-100 chromatography to afford 3 fractions, HCPS-1, HCPS-2 and The recovery rates based on crude HCPS used were 26.91%, 30.18% and 3.93% for HCPS-1, HCPS-3. HCPS-2 and HCPS-3 respectively. Based on the characterization results of HCPS by Fourier transform-infrared spectroscopy, high performance liquid chromatography and gas chromatography, we found that HCPS-3 was quite different from HCPS-1 or HCPS-2. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：422 / 429

页数：8

共 28 条

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Isolation and structural characterization of hemicelluloses from palm of Phoenix dactylifera L. [J].