The Opisthorchis felineus paramyosin:: cDNA sequence and characterization of its recombinant fragment

被引：9

作者：

Shustov, AV ^{[1
]}

Kotelkin, AT ^{[1
]}

Sorokin, AV ^{[1
]}

Ternovoi, VA ^{[1
]}

Loktev, VB ^{[1
]}

机构：

[1] State Res Ctr Virol & Biotechnol Vector, Koltsov 630559, Novosibirsk Reg, Russia

来源：

PARASITOLOGY RESEARCH | 2002年 / 88卷 / 08期

关键词：

D O I：

10.1007/s00436-001-0514-2

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The cDNA sequence of Opisthorchis felineus paramyosin (PM) was determined and shown to have 66-70% homology with two schistosomes and two cestodes. Phylogenetic analysis revealed an almost equal distance between O. felineus and these distinct clades. Because of its relatively low conservation, the PM gene may be a convenient genetic marker for studying phylogenetic relationships among platyhelminthes. A 25-kDa recombinant polypeptide corresponding to the central part of the full-length PM was produced. In Western blot analysis, murine hyperimmune serum against recombinant PM (recPM) detected 100-kDa polypeptides in the O. felineus egg and somatic antigens. Interactions of recPM with polyclonal anti-parasite antibodies and anti-recPM sera in ELISA with native antigens demonstrated that recPM carries a B cell epitope identical to the O. felineus native antigen. Our sequence and immunologic data may be helpful in developing new diagnostic tools and candidate vaccines for O. felineus infection.

引用

页码：724 / 730

页数：7

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