A dual role for the FtsK protein in Escherichia coli chromosome segregation

被引:47
作者
Capiaux, H [1 ]
Lesterlin, C [1 ]
Pérals, K [1 ]
Louarn, JM [1 ]
Cornet, F [1 ]
机构
[1] CNRS, Lab Microbiol & Genet Mol, F-31062 Toulouse, France
关键词
D O I
10.1093/embo-reports/kvf116
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
FtsK is a multifunctional protein that acts in Escherichia coli cell division and chromosome segregation. Its C-terminal domain is required for XerCD-mediated recombination between dif sites that resolve chromosome dimers formed by recombination between sister chromosomes. We report the construction and analysis of a set of strains carrying different Xer recombination sites in place of dif, some of which recombine in an FtsK-independent manner. The results show that FtsK-independent Xer recombination does not support chromosome dimer resolution. Furthermore, resolution of dimers by the Cre/loxP system also requires FtsK. These findings reveal a second role for FtsK during chromosome dimer resolution in addition to XerCD activation. We propose that FtsK acts to position the dif regions, thus allowing a productive synapse between dif sites.
引用
收藏
页码:532 / 536
页数:5
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