Crystallization and preliminary X-ray analysis of eukaryotic initiation factor 4E from Pisum sativum

被引：2

作者：

Ashby, Jamie A. ^{[2
]}

Stevenson, Clare E. M. ^{[1
]}

Maule, Andrew J. ^{[2
]}

Lawson, David M. ^{[1
]}

机构：

[1] John Innes Ctr, Dept Biol Chem, Norwich NR4 7UH, Norfolk, England

[2] John Innes Ctr, Dept Dis & Stress Biol, Norwich NR4 7UH, Norfolk, England

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2009年 / 65卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

RECESSIVE POTYVIRUS RESISTANCE; EIF4E; REVEALS; GENE;

D O I：

10.1107/S1744309109027389

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals of an N-terminally truncated 20 kDa fragment of Pisum sativum eIF4E (Delta N-eIF4E) were grown by vapour diffusion. X-ray data were recorded to a resolution of 2.2 angstrom from a single crystal in-house. Indexing was consistent with primitive monoclinic symmetry and solvent-content estimations suggested that between four and nine copies of the eIF4E fragment were possible per crystallographic asymmetric unit. eIF4E is an essential component of the eukaryotic translation machinery and recent studies have shown that point mutations of plant eIF4Es can confer resistance to potyvirus infection.

引用

页码：836 / 838

页数：3

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