Substrate stereospecificity in oxidation of (25S)-3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoyl-CoA by peroxisomal trihydroxy-5 beta-cholestanoyl-CoA oxidase

被引：29

作者：

Pedersen, JI ^{[1
]}

Veggan, T ^{[1
]}

Bjorkhem, I ^{[1
]}

机构：

[1] KAROLINSKA INST, HUDDINGE UNIV HOSP, DIV CLIN CHEM, S-14186 HUDDINGE, SWEDEN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 224卷 / 01期

关键词：

D O I：

10.1006/bbrc.1996.0981

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Partly purified 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoyl-CoA oxidase from rabbit liver peroxisomes was found to convert the 25S- but not the 25R diastereoisomer of 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestan-27-oyl-CoA into (24E)-3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholest-24-en-27-oic acid. In the presence of a peroxisomal THCA-CoA racemase, however, also the 25R isomer was oxidized. Since the mitochondrial steroid-27-hydroxylase, responsible for formation of THCA, is 25R specific a racemase seems to be obligatory for formation of cholic acid by the normal peroxisomal-dependent pathway. (C) 1996 Academic Press, Inc.

引用

页码：37 / 42

页数：6

共 27 条

[1]

BATTA AK, 1979, J LIPID RES, V20, P935

[2]

BATTA AK, 1983, J LIPID RES, V24, P94

[3]

BATTA AK, 1979, J BIOL CHEM, V254, P1907

[4] TISSUE FRACTIONATION STUDIES .17. INTRACELLULAR DISTRIBUTION OF MONOAMINE OXIDASE ASPARTATE AMINOTRANSFERASE ALANINE AMINOTRANSFERASE D-AMINO ACID OXIDASE + CATALASE IN RAT-LIVER TISSUE [J].

BAUDHUIN, P ;

BEAUFAY, H ;

SELLINGER, OZ ;

RAHMANLI, Y ;

DEDUVE, C ;

WATTIAUX, R ;

JACQUES, P .

BIOCHEMICAL JOURNAL, 1964, 92 (01) :179-+

[5]

BJORKHEM I, 1985, STEROLS BILE ACIDS, P231

[6]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[7] IDENTIFICATION OF 3-ALPHA,7-ALPHA,12-ALPHA-TRIHYDROXY-5-BETA-CHOLEST-24-ENOIC ACID AS AN INTERMEDIATE IN THE PEROXISOMAL CONVERSION OF 3-ALPHA,7-ALPHA,12-ALPHA-TRIHYDROXY-5-BETA-CHOLESTANOIC ACID TO CHOLIC-ACID [J].

FARRANTS, AKO ;

BJORKHEM, I ;

PEDERSEN, JI .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 1002 (02) :198-202

[8]

GUSTAFSSON J, 1975, J BIOL CHEM, V250, P8243

[9] BIOSYNTHESIS OF CHOLIC-ACID IN RAT-LIVER - FORMATION OF CHOLIC-ACID FROM 3ALPHA-TRIHYDROXY-5BETA-CHOLESTANOIC, 7ALPHA-TRIHYDROXY-5BETA-CHOLESTANOIC, 12-ALPHA-TRIHYDROXY-5 BETA-CHOLESTANOIC AND 3-ALPHA-5 BETA CHOLESTANOIC, 7-ALPHA-5 BETA CHOLESTANOIC, 12-ALPHA-5 BETA CHOLESTANOIC, 24-TETRAHYDROXY-5BETA-CHOLESTANOIC ACIDS [J].

GUSTAFSSON, J .

LIPIDS, 1980, 15 (02) :113-121

[10]

IKEGAWA S, 1995, BIOL PHARM BULL, V18, P1041

← 1 2 3 →