Interlaboratory comparison of methodologies for the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine

被引：34

作者：

Cooke, Marcus S. ^{[1
,2
]}

Barregard, Lars ^{[3
]}

Mistry, Vilas ^{[1
]}

Potdar, Neelam ^{[4
]}

Rozalski, Rafal ^{[5
]}

Gackowski, Daniel ^{[5
]}

Siomek, Agnieszka ^{[5
]}

Foksinski, Marek ^{[5
]}

Svoboda, Peter ^{[6
]}

Kasai, Hiroshi ^{[7
]}

Konje, Justin C.

Sallsten, Gerd ^{[3
]}

Evans, Mark D. ^{[1
]}

Olinski, Ryszard ^{[5
]}

机构：

[1] Univ Leicester, Dept Canc Studies & Mol Med, Radiat & Oxidat Stress Sect, Leicester, Leics, England

[2] Univ Leicester, Dept Genet, Leicester LE1 7RH, Leics, England

[3] Univ Gothenburg, Sahlgrenska Acad, Dept Occupat & Environm Med, Gothenburg, Sweden

[4] Univ Leicester, Dept Canc Studies & Mol Med, Reprod Sci Sect, Leicester, Leics, England

[5] Nicolaus Copernicus Univ, Coll Med Bydgoszcz, Dept Clin Biochem, Karlowicza, Poland

[6] OXOTOX Co, Veddesta Ctr, Jarfalla, Sweden

[7] Univ Occupat & Environm Hlth, Inst Ind Ecol Sci, Dept Environm Oncol, Yahatanishi Ku, Kitakyushu, Fukuoka 8555, Japan

来源：

BIOMARKERS | 2009年 / 14卷 / 02期

基金：

日本学术振兴会;

关键词：

Oxidative stress; 8-oxodGuo; ELISA; HPLC; GC; MS; urine; biomarkers; molecular epidemiology;

D O I：

10.1080/13547500802706012

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) is widely used as a marker of oxidative stress. Here we report the comparison of two, distinct chromatographic assays with an enzyme-linked immunosorbent assay (ELISA). The chromatographic assays displayed good agreement (r =:0.89, p 0.0001), whereas there was markedly worse, albeit still significant, agreement with the ELISA (high-pressure liquid chromatography followed by gas chromatography (HPLC-GC/MS), r = 0.43; HPLC with electrochemical detection (HPLC-EC), r = 0.56; p 0.0001). Mean values differed significantly between the chromatographic assays and the ELISA (HPLC-GC/MS 3.86, HPLC-EC 4.20, ELISA 18.70 ng mg-1 creatinine; p 0.0001). While it is reassuring to note good agreement between chromatographic assays, this study reveals significant short-comings in the ELISA, which brings into question its continued use in its present form.

引用

页码：103 / 110

页数：8

共 41 条

[1] Urinary mercury in people living near point sources of mercury emissions [J].

Barregard, Lars ;

Horvat, Milena ;

Mazzolai, Barbara ;

Sallesten, Gerd ;

Gibicar, Darija ;

Fajon, Vesna ;

diBona, Sergio ;

Munthe, John ;

Wangberg, Ingvar ;

Eugensson, Marie Haeger .

SCIENCE OF THE TOTAL ENVIRONMENT, 2006, 368 (01) :326-334

[2]

BESARATINIA A, 2001, CARCINOGENESIS, V223, P395

[3]

CHENG KC, 1992, J BIOL CHEM, V267, P166

[4] Urinary 8-hydroxydeoxyguano sine and urothelial carcinoma risk in low arsenic exposure area [J].

Chung, Chi-Jung ;

Huang, Chi-Jung ;

Pu, Yeong-Shiau ;

Su, Chien-Tien ;

Huang, Yung-Kai ;

Chen, Ying-Ting ;

Hsueh, Yu-Mei .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 2008, 226 (01) :14-21

[5] Evaluation of enzyme-linked immunosorbent assay and liquid chromatography-tandem mass spectrometry methodology for the analysis of 8-oxo-7,8-dihydro-2′-deoxyguanosine in saliva and urine [J].

Cooke, Marcus S. ;

Singh, Rajinder ;

Hall, Georgina K. ;

Mistry, Vilas ;

Duarte, Tiago L. ;

Farmer, Peter B. ;

Evans, Mark D. .

FREE RADICAL BIOLOGY AND MEDICINE, 2006, 41 (12) :1829-1836

[6] Measurement and meaning of oxidatively modified DNA lesions in urine [J].

Cooke, Marcus S. ;

Olinski, Ryszard ;

Loft, Steffen .

CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION, 2008, 17 (01) :3-14

[7] Novel repair action of vitamin C upon in vivo oxidative DNA damage [J].

Cooke, MS ;

Evans, MD ;

Podmore, ID ;

Herbert, KE ;

Mistry, N ;

Mistry, P ;

Hickenbotham, PT ;

Hussieni, A ;

Griffiths, HR ;

Lunec, J .

FEBS LETTERS, 1998, 439 (03) :363-367

[8] Evidence for attenuated cellular 8-oxo-7,8-dihydro-2′-deoxyguanosine removal in cancer patients [J].

Cooke, MS ;

Rozalski, R ;

Dove, R ;

Gackowski, D ;

Siomek, A ;

Evans, MD ;

Olinski, R .

BIOLOGICAL CHEMISTRY, 2006, 387 (04) :393-400

[9] Urinary 8-oxo-2′-deoxyguanosine -: Source, significance and supplements [J].

Cooke, MS ;

Evans, MD ;

Herbert, KE ;

Lunec, J .

FREE RADICAL RESEARCH, 2000, 32 (05) :381-397

[10] Oxidative DNA damage: mechanisms, mutation, and disease [J].

Cooke, MS ;

Evans, MD ;

Dizdaroglu, M ;

Lunec, J .

FASEB JOURNAL, 2003, 17 (10) :1195-1214

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