Promoter analysis of the Xanthomonas campestris pv campestris gum operon directing biosynthesis of the xanthan polysaccharide

被引：84

作者：

Katzen, F

Becker, A

Zorreguieta, A

Puhler, A

Ielpi, L

机构：

[1] FDN CAMPOMAR, INST INVEST BIOQUIM, FAC CIENCIAS EXACTAS & NAT, RA-1405 BUENOS AIRES, DF, ARGENTINA

[2] CONSEJO NACL INVEST CIENT & TECN, RA-1405 BUENOS AIRES, DF, ARGENTINA

[3] UNIV BIELEFELD, LEHRSTUHL GENET, D-33501 BIELEFELD, GERMANY

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 14期

关键词：

D O I：

10.1128/jb.178.14.4313-4318.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Xanthomonas campestris gum gene cluster is composed of 12 genes designated gumB, -C, -D, -E, -F, -G, -H, -I, -J, -K, -L, and -M. The transcriptional organization of this gene cluster was analyzed by the construction of gum-lacZ transcriptional fusions in association with plasmid integration mutagenesis. This analysis, coupled with primer extension assays, indicated that the gum region was mainly expressed as an operon from a promoter located upstream of the first gene, gumB.

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页码：4313 / 4318

页数：6

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