Efficiency of light utilization of Chlamydomonas reinhardtii under medium-duration light/dark cycles

被引:111
作者
Janssen, M
Janssen, M
de Winter, M
Tramper, J
Mur, LR
Snel, J
Wijffels, RH
机构
[1] Wageningen Univ, Dept Food Technol & Nutrit Sci, Food & Bioproc Engn Grp, NL-6700 EV Wageningen, Netherlands
[2] Univ Amsterdam, Amsterdam Res Inst Subst Ecosyst, Amsterdam, Netherlands
[3] Wageningen Univ, Lab Plant Physiol, NL-6700 EV Wageningen, Netherlands
关键词
microalgae; Chlamydomonas reinhardtii; light/dark cycles; photobioreactors; light utilization efficiency; biomass yield; quantum yield;
D O I
10.1016/S0168-1656(99)00233-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The light regime inside a photobioreactor is characterized by a light gradient with full (sun)light at the light-exposed surface and darkness in the interior of the bioreactor. Consequently, depending on the mixing characteristics, algae will be exposed to certain light/dark cycles. In this study the green alga Chlamydomonas reinhardtii was cultivated under five different light regimes: (1) continuous illumination; (2) a square-wave light/dark cycle with a light fraction (epsilon) of 0.5 and a duration (t(c)) of 6.1 s; (3) epsilon = 0.5, t(c) = 14.5 s; (4) epsilon = 0.5, t(c) = 24.3 s and (5) epsilon = 0.8, t(c) = 15.2 s. The biomass yield on light energy, protein per photons, decreased under light/dark cycles (epsilon = 0.5) in comparison to continuous light (CL), from 0.207 (CL) to 0.117-0.153 g mol(-1) (epsilon = 0.5). Concomitantly, the maximal specific photosynthetic activity, oxygen production per protein, decreased from 0.94 (CL) to 0.64-0.66 g g(-1) h (-1) (epsilon = 0.5). Also the quantum yield of photochemistry, yield of the conversion of light energy into chemical energy, decreased from 0.47 (CL) to 0.23 (epsilon = 0.5, t(c) = 24.3 s). Apparently, C. reinhardtii is not able to maintain a high photosynthetic capacity under medium-duration light/dark cycles and since specific light absorption did not change, light utilization efficiency decreased in comparison to continuous illumination. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
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页码:123 / 137
页数:15
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