2-Aminopurine-Modified Abasic-Site-Containing Duplex DNA for Highly Selective Detection of Theophylline

被引：64

作者：

Li, Minjie ^{[1
]}

Sato, Yusuke ^{[1
]}

Nishizawa, Seiichi ^{[1
]}

Seino, Takehiro ^{[1
]}

Nakamura, Kodai ^{[1
]}

Teramae, Norio ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Sci, Dept Chem, Aoba Ku, Sendai, Miyagi 9808578, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 07期

关键词：

RNA-APTAMERS; MOLECULAR DISCRIMINATION; RECOGNITION; BINDING; FLUORESCENCE; RESOLUTION; FLAVIN; ION;

D O I：

10.1021/ja8095625

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

2-Aminopurine-modified abasic-site-containing duplex [DNA 5'-TCTGC GTCCT (PX) under barT TAACG CACAC-3'/3'-AGACG CAGGA T (C) under barA ATTGC GTGTG-5'; (P) under bar = 2-aminopurine, (X) under bar = abasic site (Spacer-C3), (C) under bar = receptor base] is capable of selectively binding to the bronchodilator theophylline with a dissociation constant of 10 mu M (5 degrees C, pH 7.0, I = 0.11 M) and is applicable to monitoring serum theophylline concentrations.

引用

页码：2448 / +

页数：3

共 24 条

[1] Unusually short RNA sequences: Design of a 13-mer RNA that selectively binds and recognizes theophylline [J].

Anderson, PC ;

Mecozzi, S .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (15) :5290-5291

[2] ISOLATION OF RNA APTAMERS FOR BIOLOGICAL COFACTORS BY IN-VITRO SELECTION [J].

BURGSTALLER, P ;

FAMULOK, M .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1994, 33 (10) :1084-1087

[3] MOLECULAR RECOGNITION OF AMINO-ACIDS BY RNA-APTAMERS - AN L-CITRULLINE BINDING RNA MOTIF AND ITS EVOLUTION INTO AN L-ARGININE BINDER [J].

FAMULOK, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (05) :1698-1706

[4] DNA damage and repair [J].

Friedberg, EC .

NATURE, 2003, 421 (6921) :436-440

[5] IDENTIFICATION OF 2 NOVEL ARGININE BINDING DNAS [J].

HARADA, K ;

FRANKEL, AD .

EMBO JOURNAL, 1995, 14 (23) :5798-5811

[6]

HENDELES L, 1983, PHARMACOTHERAPY, V3, P2

[7] Biochemistry - Adaptive recognition by nucleic acid aptamers [J].

Hermann, T ;

Patel, DJ .

SCIENCE, 2000, 287 (5454) :820-825

[8] HIGH-RESOLUTION MOLECULAR DISCRIMINATION BY RNA [J].

JENISON, RD ;

GILL, SC ;

PARDI, A ;

POLISKY, B .

SCIENCE, 1994, 263 (5152) :1425-1429

[9] In vitro selection of signaling aptamers [J].

Jhaveri, S ;

Rajendran, M ;

Ellington, AD .

NATURE BIOTECHNOLOGY, 2000, 18 (12) :1293-1297

[10] Designed signaling aptamers that transduce molecular recognition to changes in fluorescence intensity [J].

Jhaveri, SD ;

Kirby, R ;

Conrad, R ;

Maglott, EJ ;

Bowser, M ;

Kennedy, RT ;

Glick, G ;

Ellington, AD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (11) :2469-2473

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