Potency Analysis of Mesenchymal Stromal Cells Using a Combinatorial Assay Matrix Approach

被引：144

作者：

Chinnadurai, Raghavan ^{[1
]}

Rajan, Devi ^{[2
]}

Qayed, Muna ^{[2
]}

Arafat, Dalia ^{[4
]}

Garcia, Marco ^{[3
]}

Liu, Yifei ^{[5
]}

Kugathasan, Subra ^{[2
]}

Anderson, Larry J. ^{[2
]}

Gibson, Greg ^{[4
]}

Galipeau, Jacques ^{[1
]}

机构：

[1] Univ Wisconsin, Carbone Comprehens Canc Ctr, Dept Med, Madison, WI 53705 USA

[2] Emory Univ, Childrens Healthcare Atlanta, Dept Pediat, Atlanta, GA 30322 USA

[3] Emory Healthcare, Atlanta, GA 30322 USA

[4] Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA

[5] Univ Wisconsin, Dept Stat, Madison, WI 53706 USA

来源：

CELL REPORTS | 2018年 / 22卷 / 09期

关键词：

STEM/STROMAL CELLS; STEM-CELLS; INTERNATIONAL-SOCIETY; CLINICAL-TRIALS; SUPPRESSION; THERAPY; INFLAMMATION; PERSPECTIVE; MODEL; CRYOPRESERVATION;

D O I：

10.1016/j.celrep.2018.02.013

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Assays that can characterize MSC immune potency need to be identified for use in advanced clinical trials. MSCs possess a number of putative regenerative and immunomodulatory properties, and an assay matrix approach may best capture involved effector pathways. We have tested two assay systems to measure the potency of MSCs derived from human subjects: MSC secretome analysis and a quantitative RNA-based array for genes specific to immunomodulatory and homing properties of MSCs. Secretome analysis identified a unique cytokine signature that is upregulated by MSCs or downregulated in responder PBMCs and correlated with T cell suppression. Use of interferon-g as a surrogate for the action of activated PBMCs on MSCs served as an alternative for the use of human PBMCs as responder cells in a potency assay. Our approach and results define and simplify the multifunctional or matrix responses of MSCs and may serve as a platform for robust potency analysis.

引用

页码：2504 / 2517

页数：14

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