Covalent display of oligosaccharide arrays in microtiter plates

被引：155

作者：

Bryan, MC

Fazio, F

Lee, HK

Huang, CY

Chang, A

Best, MD

Calarese, DA

Blixt, C

Paulson, JC

Burton, D

Wilson, IA

Wong, CH

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, Dept Mol Biol, La Jolla, CA 92037 USA

[3] Scripps Res Inst, Dept Immunol, La Jolla, CA 92037 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 28期

关键词：

D O I：

10.1021/ja048433f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A covalent array for the display of complex oligosaccharides in microtiter plates has been developed. This strategy is conducive to the display of carbohydrates to proteins of interest such as lectins and antibodies, including the broadly neutralizing antibody 2G12 against HIV envelope oligomannose and can be cleaved from the surface for further characterization by mass spectrometry. The system was used to probe the multivalent interaction of 2G12 with an optimal epitope (Kd 0.1 μM). Copyright © 2004 American Chemical Society.

引用

收藏

页码：8640 / 8641

页数：2

相关论文

共 28 条

[1] Strategies in the preparation of DNA oligonucleotide arrays for diagnostic applications [J].

Beaucage, SL .

CURRENT MEDICINAL CHEMISTRY, 2001, 8 (10) :1213-1244

[2] Saccharide display on microtiter plates [J].

Bryan, MC ;

Plettenburg, O ;

Sears, P ;

Rabuka, D ;

Wacowich-Sgarbi, S ;

Wong, CH .

CHEMISTRY & BIOLOGY, 2002, 9 (06) :713-720

[3]

Burkhart F, 2001, ANGEW CHEM INT EDIT, V40, P1274, DOI 10.1002/1521-3773(20010401)40:7<1274::AID-ANIE1274>3.0.CO

[4]

2-W

[5] Antibody domain exchange is an immunological solution to carbohydrate cluster recognition [J].

Calarese, DA ;

Scanlan, CN ;

Zwick, MB ;

Deechongkit, S ;

Mimura, Y ;

Kunert, R ;

Zhu, P ;

Wormald, MR ;

Stanfield, RL ;

Roux, KH ;

Kelly, JW ;

Rudd, PM ;

Dwek, RA ;

Katinger, H ;

Burton, DR ;

Wilson, IA .

SCIENCE, 2003, 300 (5628) :2065-2071

[6] Assembly of sugars on polystyrene plates: a new facile microarray fabrication technique [J].

Fazio, F ;

Bryan, MC ;

Lee, HK ;

Chang, A ;

Wong, CH .

TETRAHEDRON LETTERS, 2004, 45 (12) :2689-2692

[7] Synthesis of sugar arrays in microtiter plate [J].

Fazio, F ;

Bryan, MC ;

Blixt, O ;

Paulson, JC ;

Wong, CH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (48) :14397-14402

[8] Carbohydrate microarrays - a new set of technologies at the frontiers of glycomics [J].

Feizi, T ;

Fazio, F ;

Chai, WC ;

Wong, CH .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2003, 13 (05) :637-645

[9] Oligosaccharide microarrays for high-throughput detection and specificity assignments of carbohydrate-protein interactions [J].

Fukui, S ;

Feizi, T ;

Galustian, C ;

Lawson, AM ;

Chai, WG .

NATURE BIOTECHNOLOGY, 2002, 20 (10) :1011-1017

[10] Linkers and cleavage strategies in solid-phase organic synthesis and combinatorial chemistry [J].

Guillier, F ;

Orain, D ;

Bradley, M .

CHEMICAL REVIEWS, 2000, 100 (06) :2091-2157