Rapid nongenomic effects of 3,5,3′-triiodo-L-thyronine on the intracellular pH of L-6 myoblasts are mediated by intracellular calcium mobilization and kinase pathways

被引:95
作者
D'Arezzo, S
Incerpi, S
Davis, FB
Acconcia, F
Marino, M
Farias, RN
Davis, PJ
机构
[1] Univ Rome Roma Tre, Dept Biol, I-00146 Rome, Italy
[2] Univ Nacl Tucuman, CONICET, Inst Super Invest Biol, RA-4000 San Miguel De Tucuman, Argentina
[3] Vet Affairs Healthcare Network Upstate New York, Albany, NY 12208 USA
[4] Ordway Res Inst, Albany, NY 12208 USA
关键词
D O I
10.1210/en.2004-0890
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
L-T-3 and L-T-4 activated the Na+/H+ exchanger of L-6 myoblasts, with a fast nongenomic mechanism, both in the steady state and when cells undergo acid loading with ammonium chloride. Monitored with the intracellular pH-sensitive fluorescent probe 2', 7'-bis(carboxyethyl)-5(6)-carboxyfluorescein, activation of the exchanger appeared to be initiated at the plasma membrane, because T-3-agarose reproduced the effect of L-T-3, and triiodothyroacetic acid, a hormone analog previously shown to inhibit membrane actions of thyroid hormone, blocked the action of L-T-3 on the exchanger. We show here for the first time that transduction of the hormone signal in this nongenomic response requires tyrosine kinase-dependent phospholipase C activation and two different signaling pathways: 1) mobilization of intracellular calcium, assessed by the fluorescent probe fura-2, through activation of inositol trisphosphate receptors and without contributions from extracellular calcium or ryanodine receptors; and 2) protein phosphorylation involving protein kinase C and MAPK (ERK1/2), as shown by the use of kinase inhibitors and by immunoblotting for activated kinases.
引用
收藏
页码:5694 / 5703
页数:10
相关论文
共 56 条
  • [1] MODIFICATION OF L-TRIIODOTHYRONINE BINDING-SITES FROM RAT ERYTHROCYTE-MEMBRANE BY HEATING AND BY PROTEINASE TREATMENTS
    ANGEL, RC
    BOTTA, JA
    FARIAS, RN
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 897 (03) : 488 - 494
  • [2] ANGEL RC, 1989, J BIOL CHEM, V264, P19143
  • [3] AZUMA RK, 1996, AM J PHYSIOL, V270, pC585
  • [4] Inflammatory cytokines stimulate vascular smooth muscle cells locomotion and growth by enhancing α5β1 integrin expression and function
    Barillari, G
    Albonici, L
    Incerpi, S
    Bogetto, L
    Pistritto, G
    Volpi, A
    Ensoli, B
    Manzari, V
    [J]. ATHEROSCLEROSIS, 2001, 154 (02) : 377 - 385
  • [5] Effects of thyroid hormone on the neonatal renal cortical Na+/H+ antiporter
    Baum, M
    Dwarakanath, V
    Alpern, RJ
    Moe, OW
    [J]. KIDNEY INTERNATIONAL, 1998, 53 (05) : 1254 - 1258
  • [6] BERGH JJ, IN PRESS THYROID
  • [7] Bianchini L, 1997, J BIOL CHEM, V272, P271
  • [8] SOLUBILIZATION OF L-TRIIODOTHYRONINE BINDING-SITE FROM HUMAN-ERYTHROCYTE MEMBRANE
    BOTTA, JA
    FARIAS, RN
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 133 (02) : 442 - 448
  • [9] BOTTA JA, 1983, J BIOL CHEM, V258, P6690
  • [10] HEPATIC TRANSPORT-SYSTEMS REGULATING PHI, CELL-VOLUME, AND BILE SECRETION
    BOYER, JL
    GRAF, J
    MEIER, PJ
    [J]. ANNUAL REVIEW OF PHYSIOLOGY, 1992, 54 : 415 - 438