Rapid nongenomic effects of 3,5,3′-triiodo-L-thyronine on the intracellular pH of L-6 myoblasts are mediated by intracellular calcium mobilization and kinase pathways
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D'Arezzo, S
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机构:Univ Rome Roma Tre, Dept Biol, I-00146 Rome, Italy
D'Arezzo, S
Incerpi, S
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机构:Univ Rome Roma Tre, Dept Biol, I-00146 Rome, Italy
Incerpi, S
Davis, FB
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机构:Univ Rome Roma Tre, Dept Biol, I-00146 Rome, Italy
Davis, FB
Acconcia, F
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机构:Univ Rome Roma Tre, Dept Biol, I-00146 Rome, Italy
Acconcia, F
Marino, M
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机构:Univ Rome Roma Tre, Dept Biol, I-00146 Rome, Italy
Marino, M
Farias, RN
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机构:Univ Rome Roma Tre, Dept Biol, I-00146 Rome, Italy
Farias, RN
Davis, PJ
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机构:Univ Rome Roma Tre, Dept Biol, I-00146 Rome, Italy
Davis, PJ
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[1] Univ Rome Roma Tre, Dept Biol, I-00146 Rome, Italy
[2] Univ Nacl Tucuman, CONICET, Inst Super Invest Biol, RA-4000 San Miguel De Tucuman, Argentina
[3] Vet Affairs Healthcare Network Upstate New York, Albany, NY 12208 USA
L-T-3 and L-T-4 activated the Na+/H+ exchanger of L-6 myoblasts, with a fast nongenomic mechanism, both in the steady state and when cells undergo acid loading with ammonium chloride. Monitored with the intracellular pH-sensitive fluorescent probe 2', 7'-bis(carboxyethyl)-5(6)-carboxyfluorescein, activation of the exchanger appeared to be initiated at the plasma membrane, because T-3-agarose reproduced the effect of L-T-3, and triiodothyroacetic acid, a hormone analog previously shown to inhibit membrane actions of thyroid hormone, blocked the action of L-T-3 on the exchanger. We show here for the first time that transduction of the hormone signal in this nongenomic response requires tyrosine kinase-dependent phospholipase C activation and two different signaling pathways: 1) mobilization of intracellular calcium, assessed by the fluorescent probe fura-2, through activation of inositol trisphosphate receptors and without contributions from extracellular calcium or ryanodine receptors; and 2) protein phosphorylation involving protein kinase C and MAPK (ERK1/2), as shown by the use of kinase inhibitors and by immunoblotting for activated kinases.
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UNIV NACL TUCUMAN,CONSEJO NACL INVEST CIENT & TECN,INST SUPER INVEST BIOL,CHACABUCO 461,RA-4000 SAN MIGUEL TUCUMA,ARGENTINAUNIV NACL TUCUMAN,CONSEJO NACL INVEST CIENT & TECN,INST SUPER INVEST BIOL,CHACABUCO 461,RA-4000 SAN MIGUEL TUCUMA,ARGENTINA
ANGEL, RC
BOTTA, JA
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UNIV NACL TUCUMAN,CONSEJO NACL INVEST CIENT & TECN,INST SUPER INVEST BIOL,CHACABUCO 461,RA-4000 SAN MIGUEL TUCUMA,ARGENTINAUNIV NACL TUCUMAN,CONSEJO NACL INVEST CIENT & TECN,INST SUPER INVEST BIOL,CHACABUCO 461,RA-4000 SAN MIGUEL TUCUMA,ARGENTINA
BOTTA, JA
FARIAS, RN
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UNIV NACL TUCUMAN,CONSEJO NACL INVEST CIENT & TECN,INST SUPER INVEST BIOL,CHACABUCO 461,RA-4000 SAN MIGUEL TUCUMA,ARGENTINAUNIV NACL TUCUMAN,CONSEJO NACL INVEST CIENT & TECN,INST SUPER INVEST BIOL,CHACABUCO 461,RA-4000 SAN MIGUEL TUCUMA,ARGENTINA
机构:
UNIV NACL TUCUMAN,CONSEJO NACL INVEST CIENT & TECN,INST SUPER INVEST BIOL,CHACABUCO 461,RA-4000 SAN MIGUEL TUCUMA,ARGENTINAUNIV NACL TUCUMAN,CONSEJO NACL INVEST CIENT & TECN,INST SUPER INVEST BIOL,CHACABUCO 461,RA-4000 SAN MIGUEL TUCUMA,ARGENTINA
ANGEL, RC
BOTTA, JA
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UNIV NACL TUCUMAN,CONSEJO NACL INVEST CIENT & TECN,INST SUPER INVEST BIOL,CHACABUCO 461,RA-4000 SAN MIGUEL TUCUMA,ARGENTINAUNIV NACL TUCUMAN,CONSEJO NACL INVEST CIENT & TECN,INST SUPER INVEST BIOL,CHACABUCO 461,RA-4000 SAN MIGUEL TUCUMA,ARGENTINA
BOTTA, JA
FARIAS, RN
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UNIV NACL TUCUMAN,CONSEJO NACL INVEST CIENT & TECN,INST SUPER INVEST BIOL,CHACABUCO 461,RA-4000 SAN MIGUEL TUCUMA,ARGENTINAUNIV NACL TUCUMAN,CONSEJO NACL INVEST CIENT & TECN,INST SUPER INVEST BIOL,CHACABUCO 461,RA-4000 SAN MIGUEL TUCUMA,ARGENTINA