The ancient CYP716 family is a major contributor to the diversification of eudicot triterpenoid biosynthesis

被引：193

作者：

Miettinen, Karel ^{[1
,2
]}

Pollier, Jacob ^{[1
,2
]}

Buyst, Dieter ^{[3
]}

Arendt, Philipp ^{[1
,2
,4
,5
]}

Csuk, Rene ^{[6
]}

Sommerwerk, Sven ^{[6
]}

Moses, Tessa ^{[1
,2
]}

Mertens, Jan ^{[1
,2
]}

Sonawane, Prashant D. ^{[7
]}

Pauwels, Laurens ^{[1
,2
]}

Aharoni, Asaph ^{[7
]}

Martins, Jose ^{[3
]}

Nelson, David R. ^{[8
]}

Goossens, Alain ^{[1
,2
]}

机构：

[1] VIB, Dept Plant Syst Biol, Technol Pk 927, B-9052 Ghent, Belgium

[2] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium

[3] Univ Ghent, Dept Organ Chem, B-9000 Ghent, Belgium

[4] Univ Ghent, Dept Biochem & Microbiol, Lab Prot Biochem & Biomol Engn, B-9000 Ghent, Belgium

[5] VIB Med Biotechnol Ctr, B-9000 Ghent, Belgium

[6] Martin Luther Univ Halle Wittenberg, Dept Organ Chem, D-06120 Halle, Saale, Germany

[7] Weizmann Inst Sci, Dept Plant & Environm Sci, IL-76100 Rehovot, Israel

[8] Univ Tennessee, Hlth Sci Ctr, Dept Microbiol Immunol & Biochem, Memphis, TN 38163 USA

来源：

NATURE COMMUNICATIONS | 2017年 / 8卷

关键词：

GINSENOSIDE BIOSYNTHESIS; COMBINATORIAL BIOSYNTHESIS; CYTOCHROME-P450; CATALYZES; PLANTS; PROTOPANAXADIOL; DIVERSITY; EVOLUTION; SAPONINS; LICORICE;

D O I：

10.1038/ncomms14153

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Triterpenoids are widespread bioactive plant defence compounds with potential use as pharmaceuticals, pesticides and other high-value products. Enzymes belonging to the cytochrome P450 family have an essential role in creating the immense structural diversity of triterpenoids across the plant kingdom. However, for many triterpenoid oxidation reactions, the corresponding enzyme remains unknown. Here we characterize CYP716 enzymes from different medicinal plant species by heterologous expression in engineered yeasts and report ten hitherto unreported triterpenoid oxidation activities, including a cyclization reaction, leading to a triterpenoid lactone. Kingdom-wide phylogenetic analysis of over 400 CYP716s from over 200 plant species reveals details of their evolution and suggests that in eudicots the CYP716s evolved specifically towards triterpenoid biosynthesis. Our findings underscore the great potential of CYP716s as a source for generating triterpenoid structural diversity and expand the toolbox available for synthetic biology programmes for sustainable production of bioactive plant triterpenoids.

引用

页数：13

共 56 条

[1]

ProtTest: selection of best-fit models of protein evolution [J].