Application of nuclear magnetic resonance spectroscopy combined with principal component analysis in detecting inborn errors of metabolism using blood spots: a metabonomic approach

被引：47

作者：

Constantinou, MA

Papakonstantinou, E

Benaki, D

Spraul, M

Shulpis, K

Koupparis, MA

Mikros, E ^{[1
]}

机构：

[1] Univ Athens, Dept Pharm, Lab Pharmaceut Chem, GR-15771 Athens, Greece

[2] Aghia Sophia Childrens Hosp, Inst Child Hlth, Athens 11527, Greece

[3] Bruker Analyt GmbH, D-76287 Rheinstetten, Germany

[4] Univ Athens, Dept Chem, Analyt Chem Lab, GR-15771 Athens, Greece

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 511卷 / 02期

关键词：

nuclear magnetic resonance (NMR) spectroscopy; principal component analysis (PCA); inborn errors of metabolism (IEM); blood spots; phenylketonuria (PKU); maple syrup urine disease (MSUD);

D O I：

10.1016/j.aca.2004.02.012

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

NMR spectra of extracted blood spots were used to investigate the possibility for the development of a new method for mass screening concerning the diagnosis of inborn errors of metabolism (IEM). Blood spots were collected on filter papers from normal, phenylketonuric (PKU) and maple syrup urine disease (MSUD) subjects and their Carr-Purcell-Meiboom-Gill (CPMG) H-1 NMR spectra were acquired. The spectra were reduced to a number of spectral descriptors and principal component analysis (PCA) was performed. The scores plot showed that PKU and MSUD samples were well discriminated from the main cluster of points. (C) 2004 Elsevier B.V. All rights reserved.

引用

收藏

页码：303 / 312

页数：10

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