Vacuolar uptake of the phytoalexin medicarpin by the glutathione conjugate pump

被引:68
作者
Li, ZS
Alfenito, M
Rea, PA
Walbot, V
Dixon, RA
机构
[1] STANFORD UNIV,DEPT BIOL SCI,STANFORD,CA 95305
[2] SAMUEL ROBERTS NOBLE FDN INC,DIV PLANT BIOL,ARDMORE,OK 73402
关键词
Vigna radiata syn Phaseolus aureus (mung bean); Leguminosae; isoflavonoid phytoalexins; glutathione S-transferase; vacuolar uptake;
D O I
10.1016/S0031-9422(97)00031-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have studied the uptake of [H-3]-medicarpin and its glutathione conjugate(s) into vacuolar membrane vesicles from etiolated hypocotyls of mung bean (Vigna radiata). Unconjugated medicarpin is taken up at a low rate in the presence or absence of MgATP. However, [H-3]-medicarpin-glutathione conjugate(s), prepared by incubation of medicarpin with a total maize glutathione S-transferase preparation, is taken up more than four-fold faster than medicarpin in the presence of MgATP, and this uptake is MgATP-dependent. Uptake of medicarpin-glutathione was not significantly inhibited by the ionophore gramicidin-D, but was strongly inhibited by vanadate and the alternative transport substrate S-(2,4-dinitrophenyl) glutathione. Our results demonstrate, in a model system, the potential utilization of the high affinity, high capacity, uncoupler-insensitive glutathione conjugate pump for the vacuolar transport of an isoflavonoid phytoalexin. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码:689 / 693
页数:5
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