Large alkyl side-chains of isoleucine and leucine in the NPIRL region constitute the core of the vacuolar sorting determinant of sporamin precursor

被引:49
作者
Matsuoka, K [1 ]
Nakamura, K [1 ]
机构
[1] Nagoya Univ, Grad Sch Bioagr Sci, Biochem Lab, Chikusa Ku, Nagoya, Aichi 4648601, Japan
关键词
hydrophobic interaction; N-terminal propeptide; protein sorting; secretory pathway; targeting signal; vacuole;
D O I
10.1023/A:1006357413084
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The N-terminal propeptide of the sporamin precursor contains vacuolar targeting information within the Asn-26/Pro-27/Ile-28/Arg-29/Leu-30 (NPIRL) sequence. An Agrobacterium-mediated transient expression assay with tobacco BY-2 cells was employed to investigate the role of each amino acid of the NPIRL region in vacuolar targeting. Replacement of Asn-26, Pro-27, Ile-28 and Leu-30 with several amino acids caused secretion of the mutant prosporamin. Leu was the only amino acid that could be substituted for Ile-28 without affecting transport. Exchange of Leu-30 for amino acids with small side-chains abolished vacuolar delivery. These results indicate that the consensus composition of the NPIRL sequence is [preferably Asn]-[not acidic]-[Ile or Leu]-[any amino acid]-[large and hydrophobic] and suggest that the large alkyl side-chains of Ile-28 and Leu-30 constitute the core of the vacuolar sorting determinant.
引用
收藏
页码:825 / 835
页数:11
相关论文
共 45 条
[1]   Cloning and subcellular location of an Arabidopsis receptor-like protein that shares common features with protein-sorting receptors of eukaryotic cells [J].
Ahmed, SU ;
BarPeled, M ;
Raikhel, NV .
PLANT PHYSIOLOGY, 1997, 114 (01) :325-336
[2]  
BEDNAREK SY, 1991, PLANT CELL, V3, P1195, DOI 10.1105/tpc.3.11.1195
[3]   Specific accumulation of GFP in a non-acidic vacuolar compartment via a C-terminal propeptide-mediated sorting pathway [J].
Di Sansebastiano, GP ;
Paris, N ;
Marc-Martin, S ;
Neuhaus, JM .
PLANT JOURNAL, 1998, 15 (04) :449-457
[4]   DETERMINATION OF THE FUNCTIONAL ELEMENTS WITHIN THE VACUOLAR TARGETING SIGNAL OF BARLEY LECTIN [J].
DOMBROWSKI, JE ;
SCHROEDER, MR ;
BEDNAREK, SY ;
RAIKHEL, NV .
PLANT CELL, 1993, 5 (05) :587-596
[5]   Sorting of phaseolin to the vacuole is saturable and requires a short C-terminal peptide [J].
Frigerio, L ;
de Virgilio, M ;
Prada, A ;
Faoro, F ;
Vitale, A .
PLANT CELL, 1998, 10 (06) :1031-1042
[6]   The C-terminal HDEL sequence is sufficient for retention of secretory proteins in the endoplasmic reticulum (ER) but promotes vacuolar targeting of proteins that escape the ER [J].
Gomord, V ;
Denmat, LA ;
FitchetteLaine, AC ;
SatiatJeunemaitre, B ;
Hawes, C ;
Faye, L .
PLANT JOURNAL, 1997, 11 (02) :313-325
[7]   STRUCTURAL RELATIONSHIP AMONG THE MEMBERS OF A MULTIGENE FAMILY CODING FOR THE SWEET-POTATO TUBEROUS ROOT STORAGE PROTEIN [J].
HATTORI, T ;
YOSHIDA, N ;
NAKAMURA, K .
PLANT MOLECULAR BIOLOGY, 1989, 13 (05) :563-572
[8]   PROCESSING OF A PLANT VACUOLAR PROTEIN-PRECURSOR INVITRO [J].
HATTORI, T ;
ICHIHARA, S ;
NAKAMURA, K .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 166 (03) :533-538
[9]   Vacuolar storage proteins and the putative vacuolar sorting receptor BP-80 exit the Golgi apparatus of developing pea cotyledons in different transport vesicles [J].
Hinz, G ;
Hillmer, S ;
Bäumer, M ;
Hohl, I .
PLANT CELL, 1999, 11 (08) :1509-1524
[10]   PROALEURAIN VACUOLAR TARGETING IS MEDIATED BY SHORT CONTIGUOUS PEPTIDE INTERACTIONS [J].
HOLWERDA, BC ;
PADGETT, HS ;
ROGERS, JC .
PLANT CELL, 1992, 4 (03) :307-318