Extraction of lipids from Mortierella alpina and enrichment of arachidonic acid from the fungal lipids

被引：117

作者：

Zhu, M ^{[1
]}

Zhou, PP ^{[1
]}

Yu, LJ ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Sch Life Sci & Technol, Wuhan 430074, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2002年 / 84卷 / 01期

关键词：

arachidonic acid; enrichment; extraction; fungal lipids; Mortierella alpina; urea inclusion;

D O I：

10.1016/S0960-8524(02)00028-7

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Mortierella alpina is known as an arachidonic acid (AA) producing oleaginous fungus. Extraction of lipids from wet and dry M. alpina biomass was compared. Lipids yield of extraction from dry cells was higher than that of extraction from wet. Wet extraction mainly extracted lipid bodies and lipids in membranes did not extract effectively. Enrichment of AA from the fungal lipids by a urea inclusion method was studied. Most of the saturated and monounsaturated fatty acids, 93.0% and 84.6%, respectively, were removed by forming urea inclusion compounds. AA was concentrated after urea inclusion. Its content in total fatty acids increased 6.2-folds and reached 57.1% with a recovery of 81.9%. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：93 / 95

页数：3

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