Diagnosis of gastric syphilis by direct immunofluorescence staining and real-time PCR testing

被引：47

作者：

Chen, Cheng-Yen

Chi, Kai-Hua

George, Robert W.

Cox, David L.

Srivastava, Amitabh

Silva, Mario Rui

Carneiro, Fatima

Lauwers, Gregory Y.

Ballard, Ronald C.

机构：

[1] Ctr Dis Control & Prevent, Div STD Prevent, Natl Ctr HIV STD & TB Prevent, Atlanta, GA 30333 USA

[2] Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA

[3] Univ Hosp Coimbra, Dept Pathol, Coimbra, Portugal

[4] Univ Porto, Inst Mol Pathol & Immunol, P-4100 Oporto, Portugal

[5] Fac Med HS Joao, Oporto, Portugal

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 09期

关键词：

D O I：

10.1128/JCM.00721-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report on a case of gastric syphilis in a patient with chronic dyspepsia. The diagnosis was established by serology and the demonstration of spirochetes in diffusely inflammed gastric mucosa by staining with a fluorescent monoclonal antibody specific for pathogenic treponemes and by the detection of specific treponemal DNA sequences by a real-time PCR.

引用

收藏

页码：3452 / 3456

页数：5

相关论文

共 10 条

[1] The endemic treponematoses [J].

Antal, GM ;

Lukehart, SA ;

Meheus, AZ .

MICROBES AND INFECTION, 2002, 4 (01) :83-94

[2] Gastric syphilis in an HIV-infected patient [J].

Guerrero, AF ;

Straight, TM ;

Eastone, J ;

Spooner, K .

AIDS PATIENT CARE AND STDS, 2005, 19 (05) :281-285

[3]

Ikebe M, 1994, Radiat Med, V12, P171

[4] Gastric syphilis: Polymerase chain reaction detection of treponemal DNA in pseudolymphomatous lesions [J].

Inagaki, H ;

Kawai, T ;

Miyata, M ;

Nagaya, S ;

Tateyama, H ;

Eimoto, T .

HUMAN PATHOLOGY, 1996, 27 (08) :761-765

[5] SPECIFIC IMMUNOFLUORESCENT STAINING OF PATHOGENIC TREPONEMES WITH A MONOCLONAL-ANTIBODY [J].

ITO, F ;

HUNTER, EF ;

GEORGE, RW ;

POPE, V ;

LARSEN, SA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (04) :831-838

[6] New tests for syphilis:: Rational design of a PCR method for detection of Treponema pallidum in clinical specimens using unique regions of the DNA polymerase I gene [J].

Liu, H ;

Rodes, B ;

Chen, CY ;

Steiner, B .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (05) :1941-1946

[7] CROSS-REACTIVITY IN SEROLOGICAL TESTS FOR LYME-DISEASE AND OTHER SPIROCHETAL INFECTIONS [J].

MAGNARELLI, LA ;

ANDERSON, JF ;

JOHNSON, RC .

JOURNAL OF INFECTIOUS DISEASES, 1987, 156 (01) :183-188

[8] Role of polymerase chain reaction in the diagnosis of gastric syphilis [J].

Norris, SJ ;

Sell, S .

HUMAN PATHOLOGY, 1996, 27 (08) :749-750

[9] Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers [J].

Orle, KA ;

Gates, CA ;

Martin, DH ;

Body, BA ;

Weiss, JB .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (01) :49-54

[10] GASTRIC SYPHILIS - 5 RECENT CASES AND A REVIEW OF THE LITERATURE [J].

WINTERS, HA ;

NOTARFRANCESCO, V ;

BROMBERG, K ;

RAWSTROM, SA ;

VETRANO, J ;

PREGO, V ;

KUAN, J ;

RAUFMAN, JP .

ANNALS OF INTERNAL MEDICINE, 1992, 116 (04) :314-319