Acid-cleavable polymeric core-shell particles for delivery of hydrophobic drugs

被引:75
作者
Chan, Yannie
Bulmus, Volga [1 ]
Zareie, M. Hadi
Byrne, Frances L.
Barner, Leonie
Kavallaris, Maria
机构
[1] Univ New S Wales, Sch Chem Sci & Engn, CAMD, Sydney, NSW 2052, Australia
[2] Univ Technol Sydney, Fac Sci, INT, Sydney, NSW 2007, Australia
[3] Childrens Canc Inst Australia Med Res, Sydney, NSW 2031, Australia
基金
澳大利亚研究理事会; 英国医学研究理事会;
关键词
pH-sensitive particles; drug delivery; PEG grafting; dispersion polymerization; reversible addition-fragmentation chain transfer (RAFT);
D O I
10.1016/j.jconrel.2006.07.025
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Here we describe the combined use of acid-labile microgel approach and RAFT-mediated seeded dispersion polymerization technique to prepare an acid-cleavable core-shell like polymeric colloidal system for the delivery of hydrophobic drugs at slightly acidic sites. A new bisacrylate acetal crosslinker was copolymerized with n-butyl acrylate (BA) in the presence of a RAFT agent using a dispersion polymerization technique, which yielded crosslinked spherical particles with the size ranging between 150 and 500 nm. The particles were cleaved in a pH-dependent manner similar to the acid-labile hydrolysis behaviour of the crosslinker. In order to mask the hydrophobic surface of the particles, polyethylene glycol acrylate (PEG-A) was grafted onto poly(BA) seed particles via the RAFT agent groups on the particle surface. The acidic-site selective delivery potential of the poly(BA)-g-poly(PEG-A) particles was assessed in-vitro using a lipophilic fluorescent dye as a model hydrophobic drug. Ca. 73% and 34% of the total dye loaded in the particles was found to be released at pH 5.0 and 7.4 in 24 h, respectively. The growth of human neuroblastoma cells was not affected by the incubation with the core-shell particles and their cleavage by-products up to 3 mg/ml concentration. The physicochemical and the functional features support the potential value of the acid-cleavable poly(BA) core-poly(PEG-A) shell particles as carriers for the delivery of hydrophobic drugs at acidic sites. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:197 / 207
页数:11
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